| 产品编号 | bsm-61422R |
| 英文名称 | CTCF Recombinant Rabbit mAb |
| 中文名称 | 转录阻抑蛋白CTCF重组兔单抗 |
| 别 名 | Ctcf; CTCFL paralog; MRD21. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 83 kDa |
| 检测分子量 | 140 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CTCF: 680-727 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. SWISS: P49711 Gene ID: 10664 CTCF是一种结构保守、功能多样的锌指蛋白。通过不同锌指的组合，识别不同的DNA序列，并且通过自身形成多聚体或与其它蛋白相互作用、与亚细胞核结构共定位等多种方式，在基因活化与沉默、基因印记、X-染色体失活以及基因组核内空间组织等不同生理过程中发挥关键作用。CTCF在细胞生长与增殖、系统发生以及肿瘤发生等方面都有着重要的作用。 |
| 产品图片 |
Western blot analysis of (1) HeLa cell lysate; (2) Mouse brain lysate. Using CTCF (bsm-61422R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
