| 产品编号 | bsm-61766R |
| 英文名称 | IMP3 Recombinant Rabbit mAb |
| 中文名称 | 胰岛素样生长因子2mRNA结合蛋白3重组兔单抗 |
| 别 名 | CT98; IMP-3; IMP3; KOC; KOC1; VICKZ3; 2610101N11Rik; Koc13; Neilsen; mimp3; Ab2-255; RGD1306512; IF2B3_HUMAN; IGF2BP3; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer (hKOC); VICKZ family member 3; IF2B3_MOUSE; mIMP-3; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 3D10 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64 kDa |
| 检测分子量 | 69 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IMP 3: 1-200 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. SWISS: O00425 Gene ID: 10643 IGF2BP3是一种可以与胰岛素样生长因子(IGFs)结合的分泌性蛋白,其通过IGFs调节细胞的生长、分化、凋亡、黏附和运动,IGF2BP3与IGFs结合可以调节IGFs与IGF2型受体间的相互作用,改变其促有丝分裂效应、凋亡抑制效应和促细胞运动作用。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with IMP3 monoclonal antibody, unconjugated (bsm-61766R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
