| 产品编号 | bsm-61783R |
| 英文名称 | SMARCA5 Recombinant Rabbit mAb |
| 中文名称 | SMARCA5重组兔单抗 |
| 别 名 | ISWI; SNF2H; WCRF135; hISWI; hSNF2H; SMCA5_HUMAN; SMARCA5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; Sucrose nonfermenting protein 2 homolog (hSNF2H); 3.6.4.-; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 2A5 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 122 kDa |
| 检测分子量 | 122 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SNF2H: 1000-1052/1052 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. SWISS: O60264 Gene ID: 8467 解旋酶具有内在的ATP依赖性核小体重构活性。含有SmarC5的配合物能够在染色质上形成有序的核小体阵列，这可能需要完整的组蛋白H4尾部。参与在S期复制周着丝粒异染色质，特别是与BAZ1A结合。可能通过向rDNA启动子招募SIN3/HDAC1复合物来抑制rDNA位点的polI依赖性转录。也是WICH复合体的重要组成部分，是一种染色质重塑复合体，它激活核小体并将不规则染色质重新配置为规则的核小体阵列结构。 |
| 产品图片 |
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SMARCA5 antibody (bsm-61783R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
