| 产品编号 | bsm-61798R |
| 英文名称 | Sprouty 2 Recombinant Rabbit mAb |
| 中文名称 | 快速发育生长因子同源蛋白2重组兔单抗 |
| 别 名 | IGAN3; hSPRY2; sprouty2; SPY2_HUMAN; SPRY2; Spry-2; SPY2_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 12C9 |
| 交叉反应 | Human,Rat |
| 产品应用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | 42 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Spry 2: 250-315 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2.Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis. SWISS: O43597 Gene ID: 10253 Sprouty2是一类在全身组织广泛表达的软脂酰化磷蛋白,在机体的信号转导,尤其是受体蛋白对受体酪氨酸激酶(RTK)等信号通路起着重要的抑制作用,其表达在多种肿瘤组织中发生明显下降,并可进一步引起细胞异常增殖、转化及新生血管形成,从而促进肿瘤的发生及发展。 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using Sprouty 2 (bsm-61798R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Sprouty 2 antibody (bsm-61798R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
