| 产品编号 | bsm-61819R |
| 英文名称 | Phospho-GCN2 (Thr899) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化蛋白激酶GCN2蛋白重组兔单抗 |
| 别 名 | GCN2-like protein; GCN2; KIAA1338; EIF2AK4. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 187 kDa |
| 检测分子量 | 220 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human GCN2 around the phosphorylation site of T899: GHL-pT-GM(全长1649aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability.Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. SWISS: Q9P2K8 Gene ID: 440275 GCN2蛋白的作用；可能是将短期记忆变成长期记忆的一个主控调节因子， GCN2蛋白在长期记忆形成中功能的发现将可能帮助研究人员开发出提高记忆力丧失的病人提高记忆力。 |
| 产品图片 |
Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin. Using Phospho-GCN2 (Thr899) (bsm-61819R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
