| 产品编号 | bsm-61821R |
| 英文名称 | RING2 Recombinant Rabbit mAb |
| 中文名称 | 环指蛋白2重组兔单抗 |
| 别 名 | BAP-1; BAP1; DING; HIPI3; LUSYAM; RING1B; RING2; RING2_HUMAN; RNF2; Huntingtin-interacting protein 2-interacting protein 3 (HIP2-interacting protein 3); Protein DinG; RING finger protein 1B (RING1b); RING finger protein 2; RING finger protein BAP-1; RING-type E3 ubiquitin transferase RING2; 2.3.2.27; RING2_MOUSE; RING2_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 1A9 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | 38 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human RING2: 100-160/336 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes.Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation. SWISS: Q99496 Gene ID: 6045 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using RING2 (bsm-61821R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
