| 产品编号 | bsm-61914R |
| 英文名称 | MIB1 Recombinant Rabbit mAb |
| 中文名称 | E3泛素蛋白连接酶MIB1重组兔单抗 |
| 别 名 | DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6; E430019M12Rik; mindbomb; MIB1_HUMAN; MIB1; DAPK-interacting protein 1 (DIP-1); Mind bomb homolog 1; RING-type E3 ubiquitin transferase MIB1; Zinc finger ZZ type with ankyrin repeat domain protein 2; 2.3.2.27; KIAA1323; MIB1_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 110 kDa |
| 检测分子量 | 110 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MIB1: 2-53 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. SWISS: Q86YT6 Gene ID: 57534 |
| 产品图片 |
Western blot analysis of Caco2 cell lysate. Using MIB1 (bsm-61914R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
