| 产品编号 | bsm-61922R |
| 英文名称 | GRIM19 Recombinant Rabbit mAb |
| 中文名称 | 干扰素/维甲酸诱导凋亡相关基因重组兔单抗 |
| 别 名 | GRIM19; B16.6; CDA016; CGI-39; GRIM-19; MC1DN28; 2700054G14Rik; CI-B16.6; RGD1565358; NDUAD_BOVIN; NDUFA13; Cell death regulatory protein GRIM-19; Complex I-B16.6 (CI-B16.6); Gene associated with retinoic-interferon-induced mortality 19 protein (GRIM-19); NADH-ubiquinone oxidoreductase B16.6 subunit; NDUAD_HUMAN; Gene associated with retinoic and interferon-induced mortality 19 protein (GRIM-19 | Gene associated with retinoic and IFN-induced mortality 19 protein); NDUAD_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 3D11 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | 17 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NDUFA13: 60-100/144 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. SWISS: Q9P0J0 Gene ID: 51079 GRIM19是GRIMs凋亡相关基因新家族成员之一,是一种由IFN-β联合RA（干扰素/维甲酸）诱导表达的16kDa蛋白,分布在细胞核和胞浆中，GRIM-19是纯化的线粒体呼吸链复合体I的组成成分,这种复合物为ATP合成所必需的,当线粒体复合物I电子转移产生活性,GRIM-19突变体突破线粒体跨膜电位,可增强细胞对凋亡刺激的敏感性，它的过高表达可以抑制肿瘤细胞的增殖，促进细胞的凋亡。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with GRIM19 monoclonal antibody, unconjugated (bsm-61922R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
