| 产品编号 | bsm-62040R |
| 英文名称 | Cenexin1 Recombinant Rabbit mAb |
| 中文名称 | 精子尾部结构蛋白/ODF2重组兔单抗 |
| 别 名 | CT134; ODF2/1; ODF2/2; ODF84; MMTEST29; ODFP2_HUMAN; ODF2; Cenexin; Outer dense fiber of sperm tails protein 2; ODFP2_MOUSE; 84 kDa outer dense fiber protein; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 10H3 |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-2000,Flow-Cyt=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 95 kDa |
| 检测分子量 | 95 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ODF2: 800-829 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. SWISS: Q5BJF6 Gene ID: 4957 |
| 产品图片 |
Western blot analysis of Hela cell lysate. Using Cenexin1 (bsm-62040R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Cenexin1 antibody (bsm-62040R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
