| 产品编号 | bsm-62058R |
| 英文名称 | MAD3 Recombinant Rabbit mAb |
| 中文名称 | MAD3蛋白重组兔单抗 |
| 别 名 | BHLHC13; MAD3; MYX; 4631412E13Rik; Mad; zgc:66281; MAD3_HUMAN; MXD3; Max dimerizer 3; Class C basic helix-loop-helix protein 13 (bHLHc13); Max-associated protein 3; Max-interacting transcriptional repressor MAD3; MAD3_MOUSE; MAD3_RAT; MAD3_DANRE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 5H1 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | 28 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MAD3: 100-162/206 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation. SWISS: Q9BW11 Gene ID: 83463 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using MAD3 (bsm-62058R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
