| 产品编号 | bsm-62107R |
| 英文名称 | RING1 Recombinant Rabbit mAb |
| 中文名称 | 环指蛋白1重组兔单抗 |
| 别 名 | RING1A; RNF1; RING1_CANLF; RING1; Polycomb complex protein RING1; RING finger protein 1; RING-type E3 ubiquitin transferase RING1; 2.3.2.27; RING1_HUMAN; Really interesting new gene 1 protein; RING1_MOUSE; Transcription repressor Ring1A; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 12D3 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | 50 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human RING1: 140-195/406 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. SWISS: Q06587 Gene ID: 6015 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with RING1 monoclonal antibody, unconjugated (bsm-62107R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
