| 产品编号 | bsm-62144R |
| 英文名称 | MAG Recombinant Rabbit mAb |
| 中文名称 | 髓鞘相关糖蛋白-a重组兔单抗 |
| 别 名 | GMA; S-MAG; SIGLEC-4A; SIGLEC4; SIGLEC4A; SPG75; 1B236; MAG_HUMAN; MAG; MAG_MOUSE; MAG_RAT; Brain neuron cytoplasmic protein 3; Sialic acid-binding Ig-like lectin 4a (Siglec-4a); |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 11H8 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1:50-100,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | 100 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MAG: 50-100/626 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. SWISS: P20916 Gene ID: 4099 髓鞘相关糖蛋白a/b（MAG-a/b）又称：髓磷脂相关的球蛋白 髓鞘相关糖蛋白(myelin-associated glycoprotein, MAG)是免疫球蛋白超家族成员，它由中枢神经系统的少突胶质细胞和外周神经系统的施万细胞表达。 MAG定位于直接和轴突相接触的髓鞘膜的最里层，它通过介导胶质细胞与轴突的相互作用参与髓鞘的形成及其完整性的维持。同时MAG也是髓鞘来源的神经生长抑制因子的主要成分。在神经系统发育的不同阶段，MAG显示不同的功能:即发育期促进轴突生长，成熟期抑制轴突生长。其抑制作用主要由髓 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MAG monoclonal antibody, unconjugated (bsm-62144R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
