| 产品编号 | bsm-62156R |
| 英文名称 | IDH1 Recombinant Rabbit mAb |
| 中文名称 | 异柠檬酸脱氢酶1重组兔单抗 |
| 别 名 | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD; IDHC_HUMAN; IDH1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; 1.1.1.42; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 20C14 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:1000-2000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800,IP=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47 kDa |
| 检测分子量 | 45 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IDH1: 1-70/414 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. SWISS: O75874 Gene ID: 3417 IDH1又称PICD和IDP，属于异柠檬酸脱氢酶和异丙基苹果酸脱氢酶家族。这是人类原发性脑癌主要亚型的共同特征。基因变异与软骨肿瘤如内生软骨瘤或软骨肉瘤有关。Arg-132到Cys、Gly或His的突变消除了异柠檬酸到α-酮戊二酸的转化。相反，α-酮戊二酸转化为R（-）-2-羟基戊二酸。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with IDH1 monoclonal antibody, unconjugated (bsm-62156R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
