| 产品编号 | bsm-62197R |
| 英文名称 | SMCHD1 Recombinant Rabbit mAb |
| 中文名称 | SMCHD1蛋白重组兔单抗 |
| 别 名 | SMHD1_HUMAN; SMCHD1; SMC hinge domain-containing protein 1; 3.6.1.-; KIAA0650; SMHD1_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 8A7 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 226 kDa |
| 检测分子量 | 226 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SMCHD1: 1800-2005/2005 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture.Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments.Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin. SWISS: A6NHR9 Gene ID: 23347 |
| 产品图片 |
Western blot analysis of 293T cell lysate. Using SMCHD1 (bsm-62197R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with SMCHD1 monoclonal antibody, unconjugated (bsm-62197R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
