| 产品编号 | bsm-62202R |
| 英文名称 | PDZK1 Recombinant Rabbit mAb |
| 中文名称 | PDZ结构域PDZK1蛋白重组兔单抗 |
| 别 名 | CAP70; CLAMP; NHERF-3; NHERF3; PDZD1; 1700023D20Rik; 2610507N21Rik; 4921513F16Rik; D3Ertd537e; mPDZK1; naPi-Cap1; NHRF3_HUMAN; PDZK1; CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1 (NaPi-Cap1); PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3; NHRF3_MOUSE; NHRF3_RABIT; NHRF3_RAT; C-terminal-linking and modulating protein; Dietary Pi-regulated RNA-1; Diphor-1; Diphor1; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 3A11 |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57 kDa |
| 检测分子量 | 70 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PDZK1: 450-519/519 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with NHERF1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. SWISS: Q5T2W1 Gene ID: 5174 |
| 产品图片 |
Western blot analysis of T47-D cell lysate. Using PDZK1 (bsm-62202R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDZK1 Monoclonal Antibody, Unconjugated (bsm-62202R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
