| 产品编号 | bsm-62263R |
| 英文名称 | MCM4 Recombinant Rabbit mAb |
| 中文名称 | 微小染色体维持缺陷蛋白4重组兔单抗 |
| 别 名 | CDC21; CDC54; IMD54; NKCD; NKGCD; P1-CDC21; hCdc21; 19G; Mcmd4; mKIAA4003; mcdc21; MCM4_HUMAN; MCM4; CDC21 homolog; 3.6.4.12; MCM4_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 7F9 |
| 交叉反应 | Human (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 97 kDa |
| 检测分子量 | 97 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MCM4: 820-863/863 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. SWISS: P33991 Gene ID: 4173 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MCM4 monoclonal antibody, unconjugated (bsm-62263R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MCM4) monoclonal Antibody, unconjugated (bsm-62263R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
