| 产品编号 | bsm-62268R |
| 英文名称 | GSK3A Recombinant Rabbit mAb |
| 中文名称 | 糖原合酶激酶3α重组兔单抗 |
| 别 名 | 2700086H06Rik; GSK3A_HUMAN; GSK3A; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; 2.7.11.26; GSK3A_MOUSE; GSK3A_RAT; Factor A (FA); |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 4G4 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | 51 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human GSK 3 alpha: 50-100 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1.Requires primed phosphorylation of the majority of its substrates. SWISS: P49840 Gene ID: 2931 组成性活性蛋白激酶，通过磷酸化和失活糖原合成酶（GYS1或GYS2）、CTNNB1/β连环蛋白、APC和AXIN1，在激素控制葡萄糖稳态、Wnt信号传导和转录因子及微管的调节中作为负调节因子。它的大多数底物需要启动磷酸化。通过磷酸化和抑制GYS1活性从而促进糖原合成的胰岛素调节。调节肝脏的糖原代谢，而不是肌肉。也可能通过调节转录因子的激活来介导胰岛素抵抗的发展。在Wnt信号中，调节核CTNNB1/β连环蛋白的水平和转录活性。促进淀粉样前体蛋白（APP）的加工和阿尔茨海默病APP源性淀粉样斑块的形成。可能参 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with GSK3A monoclonal antibody, unconjugated (bsm-62268R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-GSK3A antibody (bsm-62268R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
