| 产品编号 | bsm-62339R |
| 英文名称 | Neurocan Recombinant Rabbit mAb |
| 中文名称 | 神经粘蛋白重组兔单抗 |
| 别 名 | CSPG3; NCAN_HUMAN; NCAN; Chondroitin sulfate proteoglycan 3; NEUR; NCAN_RAT; 245 kDa early postnatal core glycoprotein; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 5C1 |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 143 kDa |
| 检测分子量 | 130-270 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Neurocan: 2-51/1321 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. SWISS: O14594 Gene ID: 1463 神经生物学相关蛋白（Neurobiology） 神经粘蛋白(Neurocan)是由神经元分泌的特异性蛋白质,广泛分布于神经系统,可与神经元细胞膜上特异性受体神经粘附分子和神经元胶质细胞黏附分子结合,调节细胞连接,影响轴突生长.而在中枢神经系统损伤后,Neurocan的表达增加,并抑制轴突生长.Neurocan在中枢神经系统的形成和损伤后的修复都扮演着重要的角色. |
| 产品图片 |
Western blot analysis of Fetal brain cell lysate. Using Neurocan (bsm-62339R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Neurocan Monoclonal Antibody, Unconjugated (bsm-62339R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Neurocan Monoclonal Antibody, Unconjugated (bsm-62339R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
