| 产品编号 | bsm-62342R |
| 英文名称 | Nm23-H2/NME2 Recombinant Rabbit mAb |
| 中文名称 | 肿瘤抑制基因NME2重组兔单抗 |
| 别 名 | CNDPK; NDPK2; NDKB; NM23-C2; NM23B; NDPK-B; NDPKB; NM23-H2; PUF; NM23-LV; NMELV; nm23-M2; nm23-2; p18-12d; NDKB_BOVIN; NME2; NDK B; NDP kinase B; Histidine protein kinase NDKB; 2.7.4.6; NDKB_CANLF; NDKB_HUMAN; C-myc purine-binding transcription factor PUF; NDKB_MOUSE; P18; NDKB_PIG; NDKB_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 8D6 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | 17 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NDK B: 100-152/152 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NM23 nucleoside diphosphate kinase A. Major role in the synthesis of nucleoside triphosphates other than ATP. Holoenzyme is a hexamer of the A and B subunits. Mutations have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been described. SWISS: P22392 Gene ID: 654364 Nm23-H2在高转移性的肿瘤中呈现低表达，提示NM23-H2具有转移抑制的功能。其抑制肿瘤转移机制为增强微丝、微管的聚合，影响细胞的移动，从而抑制肿瘤的侵袭和转移。 |
| 产品图片 |
Western blot analysis of LnCaP cell lysate. Using Nm23-H2/NME2 (bsm-62342R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The 293T (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Nm23-H2/NME2 antibody (bsm-62342R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
