| 产品编号 | bsm-62379R |
| 英文名称 | SMG1 Recombinant Rabbit mAb |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶Smg1重组兔单抗 |
| 别 名 | 61E3.4; ATX; LIP; 2610207I05Rik; 5430435M13Rik; C130002K18Rik; mKIAA0421; SMG1_HUMAN; SMG1; SMG-1; hSMG-1; Lambda/iota protein kinase C-interacting protein (Lambda-interacting protein); Nonsense mediated mRNA decay-associated PI3K-related kinase SMG1; 2.7.11.1; KIAA0421; SMG1_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 411 kDa |
| 检测分子量 | 410 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SMG1: 3600-3661 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. SWISS: Q96Q15 Gene ID: 23049 |
| 产品图片 |
Western blot analysis of Saos2 cell lysate. Using SMG1 (bsm-62379R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
