| 产品编号 | bsm-62650R |
| 英文名称 | TPMT Recombinant Rabbit mAb |
| 中文名称 | 硫嘌呤甲基转移酶重组兔单抗 |
| 别 名 | TPMTD; TPMT_HUMAN; TPMT; Thiopurine methyltransferase; 2.1.1.67; TPMT_MOUSE; TPMT_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 1E9 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:100-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | 30 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TPMT: 101-125 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. SWISS: P51580 Gene ID: 7172 TPMT是硫嘌呤类药物（如硫唑嘌呤、巯嘌呤和硫鸟嘌呤）的代谢中最主要的酶之一，起到的作用是在这类化合物的硫原子上增加一个甲基；这个过程中提供甲基的是S-腺苷甲硫氨酸，后者同时被转化成S-腺苷-L-高半胱氨酸。 TPMT基因的缺陷会让人体无法将这类药物灭活，未代谢的药物因此在体内大量累积，从而引起严重甚至致命的骨髓抑制，表现为贫血、血小板减少症（导致出血）和白细胞减少症（导致感染）等. |
| 产品图片 |
Western blot analysis of Jurkat cell lysate. Using TPMT (bsm-62650R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
