| 产品编号 | bsm-62684R |
| 英文名称 | UBE2D1 Recombinant Rabbit mAb |
| 中文名称 | 泛素蛋白连接酶D1重组兔单抗 |
| 别 名 | E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A; UB2D1_HUMAN; UBE2D1; (E3-independent) E2 ubiquitin-conjugating enzyme D1; E2 ubiquitin-conjugating enzyme D1; Stimulator of Fe transport (SFT); UBC4/5 homolog; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1; 2.3.2.23; UBC5A; UB2D1_MOUSE; UB2D1_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 12G8 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:100-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | 17 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human UBE2D1: 110-147 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. SWISS: P51668 Gene ID: 7321 |
| 产品图片 |
Western blot analysis of Raji cell lysate. Using UBE2D1 (bsm-62684R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
