| 产品编号 | bsm-62722R |
| 英文名称 | TAF3 Recombinant Rabbit mAb |
| 中文名称 | TAF3重组兔单抗 |
| 别 名 | TAF3_HUMAN; TAF3; 140 kDa TATA box-binding protein-associated factor; TBP-associated factor 3; Transcription initiation factor TFIID 140 kDa subunit (TAF(II)140 | TAF140 | TAFII-140 | TAFII140); TAF140; TAFII-140; TAFII140; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 11G7 |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 104 kDa |
| 检测分子量 | 150 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TAF3: 201-370 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription.TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex. SWISS: Q5VWG9 Gene ID: 83860 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using TAF3 (bsm-62722R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
