| 产品编号 | bsm-62741R |
| 英文名称 | phospho-PBK (Thr9) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化PDZ连接激酶/T-LAK细胞源蛋白激酶重组兔单抗 |
| 别 名 | PBK (phospho-T9); p-PBK; phospho-PBK; PBK (phospho-Thr9); CT84; HEL164; Nori-3; SPK; TOPK; TOPK_HUMAN; PBK; Cancer/testis antigen 84 (CT84); MAPKK-like protein kinase; PDZ-binding kinase; Spermatogenesis-related protein kinase (SPK); T-LAK cell-originated protein kinase; 2.7.12.2; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 9C3 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | 40 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PBK around the phosphorylation site of T9: FK-pT-PS |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. SWISS: Q96KB5 Gene ID: 55872 PBK/TOPK是一种新近发现的丝-苏氨酸激酶,属于MAPKK分子家族成员，具有参与调控恶性肿瘤细胞的增殖和周期变化, 促进肿瘤细胞转化, 并且通过MAPKK信号通路参与调控细胞DNA损伤修复. |
| 产品图片 |
Hela (H) cells were treated with or without Nocodazole, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PBK (Thr9) monoclonal antibody, unconjugated (bsm-62741R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PBK (Thr9) Monoclonal Antibody, Unconjugated (bsm-62741R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PBK (Thr9) Monoclonal Antibody, Unconjugated (bsm-62741R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PBK (Thr9) Monoclonal Antibody, Unconjugated (bsm-62741R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
