| 产品编号 | bsm-62823R |
| 英文名称 | TBP7 Recombinant Rabbit mAb |
| 中文名称 | Tat结合蛋白7重组兔单抗 |
| 别 名 | MIP224; RPT3; S6; TBP-7; TBP7; CK820_G0030442; CIP21; psmc4; wu:fe38b10; zgc:63709; PRS6B_BOVIN; 26S proteasome AAA-ATPase subunit RPT3; Proteasome 26S subunit ATPase 4; PRS6B_HUMAN; MB67-interacting protein; Tat-binding protein 7 (TBP-7); PRS6B_MOUSE; PRS6B_RAT; Proteasome 26S subunit ATPase 4 (S6 ATPase); |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 11G7 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47 kDa |
| 检测分子量 | 51 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PSMC4: 350-418 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. SWISS: P43686 Gene ID: 5704 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with TBP7 monoclonal antibody, unconjugated (bsm-62823R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-TBP7 antibody (bsm-62823R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
