扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-AQP3  antibody (bs-1253R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1253R
英文名称 AQP3
中文名称 水通道蛋白-3抗体
别    名 AQP 3; AQP3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE.  
Specific References  (8)     |     bs-1253R has been referenced in 8 publications.
[IF=7.675] Yimeng Fan. et al. Pingwei San Ameliorates Spleen Deficiency-Induced Diarrhea through Intestinal Barrier Protection and Gut Microbiota Modulation. ANTIOXIDANTS-BASEL. 2023 May;12(5):1122  IHC ;  Rat.  
[IF=4.331] Tang SC et al. Glycolic acid attenuates UVB-induced aquaporin-3, matrix metalloproteinase-9 expression, and collagen degradation in keratinocytes and mouse skin. Biochem J. 2019 May 21;476(10):1387-1400.  WB ;  Human.  
[IF=4.24] Zhang, Haifeng, et al. "The AQP-3 water channel and the ClC-3 chloride channel coordinate the hypotonicity-induced swelling volume in nasopharyngeal carcinoma cells." The International Journal of Biochemistry & Cell Biology (2014).  IP ;  Human.  
[IF=3.887] Huijun LI. et al. Screening the effective components in treating dampness stagnancy due to spleen deficiency syndrome and elucidating the potential mechanism of Poria water extract. CHIN J NAT MEDICINES. 2023 Feb;21:83  WB ;  Rat.  
[IF=3.414] Li X et al. Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge. In loperamide-induced rats. J Ethnopharmacol. 2019 Aug 10;240:111961.  WB ;  Rat.  
[IF=2.826] Takanori Watanabe et al. Aquaporin 3 Expression in Endometrioid Carcinoma of the Uterine Body Correlated With Early Stage and Lower Grade. Pathol Oncol Res. 2020 Oct;26(4):2247-2253.  IHC ;  Human.  
[IF=2.311] Haifa Alkhalifa. et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett. 2021 Jun;21(6):1-11  WB ;  Human.  
[IF=1.935] Sato K et al. Different Prognostic Implications of Aquaporin-1 and Aquaporin-5 Expression among Different Histological Types of Ovarian Carcinoma.Pathol Oncol Res. 2018 Jul 19.  IHC-P ;  Human.  
研究领域 肿瘤  信号转导  通道蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human (predicted: Rat,Mouse)
产品应用 WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 32kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse AQP3: 201-292/292 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

Function:
Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
Q8R2N1

Gene ID:
11828

Database links:

Entrez Gene: 360 Human

Entrez Gene: 11828 Mouse

Entrez Gene: 65133 Rat

Omim: 600170 Human

SwissProt: Q92482 Human

SwissProt: Q8R2N1 Mouse

SwissProt: P47862 Rat

Unigene: 234642 Human

Unigene: 34043 Mouse

Unigene: 11109 Rat



通道蛋白(Channel Protein)
AQP 3主要分布于肾脏集合管上皮细胞基侧质膜,除了对水有通透性外、还可转运尿素和甘油等小分子物质,而尿素在尿液浓缩机制中有着重要的作用.
有学者认为:AQP-3的阳性率与某些肿瘤分化程度有关(胃癌、结肠癌、乳腺癌、前列腺癌、子宫癌、卵巢癌、甲状腺癌等)
产品图片
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):A431.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版权所有 2004-2026 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    认证编号: 00122Q31509R1M/1100
京ICP备05066980号-1         京公网安备110107000727号