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Rabbit Anti-Aquaporin 4  antibody (bs-0634R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0634R
英文名称 Aquaporin 4
中文名称 水通道蛋白4抗体
别    名 aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4.  
Specific References  (9)     |     bs-0634R has been referenced in 9 publications.
[IF=7.675] Yimeng Fan. et al. Pingwei San Ameliorates Spleen Deficiency-Induced Diarrhea through Intestinal Barrier Protection and Gut Microbiota Modulation. ANTIOXIDANTS-BASEL. 2023 May;12(5):1122  IHC ;  Rat.  
[IF=6.42] Cao et al. Hypertonic saline reduces lipopolysaccharide-induced mouse brain edema through inhibiting aquaporin 4 expression. (2012) Crit.Care. 16:R186  WB/FC ;  Mouse.  
[IF=3.887] Huijun LI. et al. Screening the effective components in treating dampness stagnancy due to spleen deficiency syndrome and elucidating the potential mechanism of Poria water extract. CHIN J NAT MEDICINES. 2023 Feb;21:83  WB ;  Rat.  
[IF=3.252] Ganggang Li. et al. Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells. Brain Res. 2022 Jan;1774:147723  WB ;  Rat.  
[IF=2.826] Takanori Watanabe et al. Aquaporin 3 Expression in Endometrioid Carcinoma of the Uterine Body Correlated With Early Stage and Lower Grade. Pathol Oncol Res. 2020 Oct;26(4):2247-2253.  IHC ;  Human.  
[IF=2.51] Gang, Zhang, et al. "Detection of hypoxic-ischemic brain injury with 3D-enhanced T2* weighted angiography (ESWAN) imaging." European Journal of Radiology (2013).  IHC-P ;  Pig.  
[IF=2.02] Duan, Hubin, et al. "The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury." Journal of Surgical Research (2013).  IHC-P ;  Rat.  
[IF=0.64] Rajesh, Kumar, Li Xiaojie, and Kong Xiangying. "The Effect of Early Intervention and Rehabilitation in the Expression of Aquaporin-4; and Ultrastructure Changes on Rats Offsprings Damaged Brain Caused by Intrauterine Infection." Journal of Korean Neurosurgical Society 58.1 (2015): 14-21.  WB ;  Rat.  
[IF=0.23] Li, Cheng, et al. "Melatonin lowers edema after spinal cord injury." Neural Regeneration Research 9.24 (2014): 2205.  IHC-P ;  Rat.  
研究领域 肿瘤  信号转导  通道蛋白  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Pig,Cow,Rabbit,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 36kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human AQP4: 271-323/323 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Subunit:
Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Brain, muscle, heart, kidney, lung, and trachea.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
P55087

Gene ID:
361

Database links:

Entrez Gene: 281008 Cow

Entrez Gene: 361 Human

Entrez Gene: 11829 Mouse

Entrez Gene: 25293 Rat

Omim: 600308 Human

SwissProt: O77750 Cow

SwissProt: P55087 Human

SwissProt: P55088 Mouse

SwissProt: P47863 Rat

Unigene: 281008 Cow

Unigene: 315369 Human

Unigene: 250786 Mouse

Unigene: 90091 Rat



通道蛋白(Channel Protein)
水通道蛋白(AQPs)广泛分布于机体不同组织器官中,脑组织中的水通道蛋白主要为AQP4.
AQP-4蛋白主要在星型胶质细胞和室管膜细胞内表达,尤其在于毛细血管和软脑膜直接接触的胶质细胞上及血管周表达丰富.
水通道蛋白4的分布特点与其功能密切相关,可能是脑脊液重吸收、渗透压调节、脑水肿形成等生理、病理过程的分子生物学基础.研究水通道蛋白4可为脑水肿及水代谢疾病提供分子水平的理论依据,同时为临床治疗脑水肿及水代谢疾病提供一种新途径.
产品图片
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Polyclonal Antibody, Unconjugated (bs-0634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Polyclonal Antibody, Unconjugated (bs-0634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: K562 (blue).
Primary Antibody:Rabbit Anti-Aquaporin 4 antibody (bs-0634R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0634R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-AQP 4 antibody (bs-0634R-A488)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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