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Rabbit Anti-Bcl-2 antibody (bs-0032R)
~~~促销,代码SQ220725~~~
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产品编号 bs-0032R
英文名称 Bcl-2
中文名称 Bcl-2抗体
别    名 Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.  
Specific References  (128)     |     bs-0032R has been referenced in 128 publications.
[IF=4.36] Zhuohong Li. et al. Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice. J Ethnopharmacol. 2022 Mar;:115158  WB ;  Mouse.  
[IF=2.248] Wang Yulong. et al. LncRNA GAS5 promotes abdominal aortic aneurysm formation through regulating the miR-185-5p/ADCY7 axis. Anti-Cancer Drug. 2022 Mar;33(3):225-234  WB ;  Human.  
[IF=0] Han et al. Protective effects of tao-Hong-si-wu decoction on memory impairment and hippocampal damage in animal model of vascular dementia. (2015) Evid.Based.Complement.Alternat.Me. 2015:195835  IHC-P ;  Rat.  
[IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.  IHC-P + WB ;  Mouse.  
[IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  WB ;  Mouse.  
[IF=3.061] Zhu H et al. Catalpol improves axonal outgrowth and reinnervation of injured sciatic nerve by activatingAkt/mTOR pathway and regulating BDNF and PTEN expression. Am J Transl Res. 2019 Mar 15;11(3):1311-1326.  WB ;  Mouse.  
[IF=3.06] Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192.  WB ;  Human.  
[IF=3.332] Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22.  WB ;  Human.  
[IF=4.171] Zhao Y et al. Walnut Polyphenol Extract Protects against Malathion- and Chlorpyrifos-Induced Immunotoxicity by Modulating TLRx-NOX-ROS. Nutrients. 2020 Feb 27;12(3). pii: E616.  WB ;  Mouse.  
[IF=3.212] Yi Zhuet al. MicroRNA MiR-27a-5p Alleviates the Cerulein-Induced Cell Apoptosis and Inflammatory Injury of AR42J Cells by Targeting Traf3 in Acute Pancreatitis. Inflammation . 2020 Oct;43(5):1988-1998.  WB ;  rat.  
[IF=4.679] Haiyang Zhang. et al. Lycopene ameliorates chronic stress-induced hippocampal injury and subsequent learning and memory dysfunction through inhibiting ROS/JNK signaling pathway in rats. Food Chem Toxicol. 2020 Nov;145:111688  WB ;  Rat.  
[IF=2.823] Dan Liu. et al. Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency. Mol Reprod Dev. 2020 Aug;87(8):887-898  IHC ;  Mouse.  
[IF=4.225] Ganggang Ma. et al. Blockade of TRPM7 Alleviates Chondrocyte Apoptosis and Articular Cartilage Damage in the Adjuvant Arthritis Rat Model Through Regulation of the Indian Hedgehog Signaling Pathway. Front Pharmacol. 2021; 12: 655551  WB ;  Rat.  
[IF=2.842] Xiao-yu Liang. et al. Astragaloside IV Alleviates Liver Inflammation, Oxidative Stress and Apoptosis to Protect Against Experimental Non-Alcoholic Fatty Liver Disease. Diabet Metab Synd Ob. 2021; 14: 1871–1883  WB ;  Human.  
[IF=6.792] Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319  WB ;  Quail.  
[IF=14.593] Guo-Bin Ding. et al. Molecularly engineered tumor acidity-responsive plant toxin gelonin for safe and efficient cancer therapy. Bioact Mater. 2022 Feb;:  IHC ;  Mouse.  
[IF=6.543] Zhou Wan. et al. The Effects of RBP4 and Vitamin D on the Proliferation and Migration of Vascular Smooth Muscle Cells via the JAK2/STAT3 Signaling Pathway. Oxid Med Cell Longev. 2022;2022:3046777  WB ;  Rat.  
[IF=6.291] Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166  WB ;  Bovine.  
[IF=4.432] Yujie Liu. et al. Protective effects of hydroxy-α-sanshool from the pericarp of Zanthoxylum bungeanum Maxim. On D-galactose/AlCl3-induced Alzheimer's disease-like mice via Nrf2/HO-1 signaling pathways. Eur J Pharmacol. 2022 Jan;914:174691  IHC ;  Mouse.  
[IF=3.105] Zheng Min. et al. Combined Usage of Trimetazidine With 3-Bromopyruvate May Lead to Cardiotoxicity by Activating Oxidative Stress and Apoptosis in Rats. J Cardiovasc Pharm. 2021 Dec;78(6):819-825  IHC ;  Rat.  
[IF=4.35] Xin Fan. et al. Anti-Colon Cancer Activity of Novel Peptides Isolated from In Vitro Digestion of Quinoa Protein in Caco-2 Cells. Foods. 2022 Jan;11(2):194  WB ;  Human.  
[IF=2.005] Honghong He. et al. Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF-1 and VEGF. 2021 Dec 30  WB ;  Yak.  
[IF=5.005] Haijin Liu. et al. Chicken ISG12(2) attenuates Newcastle disease virus and enhances the efficiency of Newcastle disease vaccine via activating immune pathways. 2021 Dec 13  WB ;  Chicken.  
[IF=6.291] Jia Cui. et al. Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits. Ecotox Environ Safe. 2021 Dec;227:112922  IHC ;  Rabbit.  
[IF=4.432] Jinni Meng. et al. Dehydrocostuslactone attenuated oxygen and glucose deprivation/reperfusion-induced PC12 cell injury through inhibition of apoptosis and autophagy by activating the PI3K/AKT/mTOR pathway. Eur J Pharmacol. 2021 Nov;911:174554  WB ;  Rat.  
[IF=4.872] Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:112350  WB ;  Duck.  
[IF=4.268] Xiping Yang. et al. The piperine derivative HJ105 inhibits Aβ1-42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis. Phytomedicine. 2021 Apr;:153571  WB ;  Rat.  
[IF=2.094] Dongmei Jiang. et al. Spermidine at supraphysiological doses induces oxidative stress and granulosa cell apoptosis in mouse ovaries. Theriogenology. 2021 Apr;:  WB ;  Mouse.  
[IF=6.792] Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319  WB ;  Quail.  
[IF=9.986] Jiyoung Hwang. et al. SOD1 suppresses pro-inflammatory immune responses by protecting against oxidative stress in colitis. Redox Biol. 2020 Oct;37:101760  WB ;  Human.  
[IF=2.886] Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341–8354  ICC ;  Rat, Human.  
[IF=1.698] Sheng-Tao Ling. et al. Hydroxychloroquine Blocks Autophagy and Promotes Apoptosis of the Prostate after Castration in Rats. Urol Int. 2020;104(11-12):968-974  IHC ;  Rat.  
[IF=4.966] Xiang Sun. et al. Tristetraprolin destabilizes NOX2 mRNA and protects dopaminergic neurons from oxidative damage in Parkinson's disease. Faseb J. 2020 Nov;34(11):15047-15061  WB ;  Mouse.  
[IF=3.375] Yingdong Lu. et al. Qiliqiangxin prevents right ventricular remodeling by inhibiting apoptosis and improving metabolism reprogramming with pulmonary arterial hypertension. Am J Transl Res. 2020; 12(9): 5655–5669  IHC ;  Rat.  
[IF=2.795] Jiejie Yang. et al. TMEM166 inhibits cell proliferation, migration and invasion in hepatocellular carcinoma via upregulating TP53. Mol Cell Biochem. 2021 Feb;476(2):1151-1163  WB ;  Human.  
[IF=6.304] Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14  WB ;  Bovine.  
[IF=4.872] Muhammad Fakhar-e-Alam Kulyar. et al. Cluster of differentiation 147 (CD147) expression is linked with thiram induced chondrocyte’s apoptosis via Bcl-2/Bax/Caspase-3 signalling in tibial growth plate under chlorogenic acid repercussion. Ecotox Environ Safe. 2021 Apr;213:112059  WB,IF ;  Chicken.  
[IF=5.201] Xuemei Shen. et al. CircINSR Regulates Fetal Bovine Muscle and Fat Development. Front Cell Dev Biol. 2020; 8: 615638  WB ;  Bovine.  
[IF=5.014] Chen-Yen Chienet al. Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats. Antioxidants (Basel) . 2020 Jul 29;9(8):679.  WB, IHC ;  rat.  
[IF=3.69] Lei Yuet al. TMF, a natural dihydroflavonoid isolated from Scutellaria javanica Jungh, stimulates anticancer activity of s180 cancer-bearing mice, induces apoptosis, inhibits invasion and migration on HepG-2 cells. J Ethnopharmacol . 2020 Dec 5;263:113072.  WB ;  Human.  
[IF=3.647] Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci . 2020 Oct 1;258:118147.  WB ;  Human.  
[IF=2.733] Kai-Li Liu et al. Effects of seawater immersion on open traumatic brain injury in rabbit model. Brain Res. 2020 Sep 15;1743:146903.  WB/IHC/IF ;  Rabbit.  
[IF=3.361] Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383.  IHC,WB ;  rat.  
[IF=2.407] Zhang M et al. Cognitive-enhancing effects of fibrauretine on Aβ1–42-induced Alzheimer's disease by compatibilization with ginsenosides. Neuropeptides. 2020 Jan 20:102020.  WB&IHC ;  Mouse.  
[IF=2.666] X Zhou et al. Modulating NMDA receptors to treat MK-801-induced schizophrenic cognition deficit: effects of clozapine combining with PQQ treatment and possible mechanisms of action. BMC Psychiatry. 2020 Mar 6;20(1):106.  WB ;  rat.  
[IF=2.154] Wang Y et al. H2S mediates apoptosis in response to inflammation through PI3K/Akt/NFκB signaling pathway. Biotechnol Lett. 2019 Dec 23.  WB ;  bovine.  
[IF=1.851] Wu Y et al. Hydroxypropyl‑β‑cyclodextrin attenuates the epithelial‑to‑mesenchymal transition via endoplasmic reticulum stress in MDA‑MB‑231 breast cancer cells. Mol Med Rep. 2019 Nov 6.  WB ;  Human.  
[IF=3.743] Liu F et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019 Oct 18;120:109533.  ICF ;  Human.  
[IF=4.868] Feng Y et al. Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway. Oxid Med Cell Longev. 2019 Nov 6;2019:7067619.  IHC-P&WB ;  Mouse&Human.  
[IF=1.07] Wei X et al. c [RGDyk]-coated liposomes loaded with adriamycin and miR-21 mimics inhibit the growth of hepatoma cell line SMCC-7721 via up-regulating Bax and p53. Transl Cancer Res 2019;8(4):1311-1318.  WB ;  Human.  
[IF=2.589] Yin G et al. Effect of 5-aminolevulinic acid photodynamic therapy on the expression of apoptosis inhibitors Bcl-2 and Survivin in keratinocytes of condyloma acuminatum . Photodiagnosis Photodyn Ther. 2019 Jul 23. pii: S1572-1000(19)30016-X.  IHC ;  Human.  
[IF=3.571] Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27.  WB&IHC-P ;  Rat.  
[IF=0.181] Zhu HF et al. Catalpol enhances neurogenesis in the SVZ in a rat model of strokes. Int J Clin Exp Med 2019;12(7):8068-8077.  WB ;  Rat.  
[IF=3.775] Chen Z et al. Final-2 targeted glycolysis mediated apoptosis and autophagy in human lung adenocarcinoma cells but failed to inhibit xenograft in nude mice. Food Chem Toxicol. 2019 Aug;130:1-11.  WB ;  Human.  
[IF=3.288] Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223.  IHC-P ;  Human.  
[IF=3.44] Chen B et al. Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.(2018) Brain Res Bull.  WB ;  Mouse.  
[IF=0] Hermawati E et al. Spatial Memory Disturbance Following Transient Brain Ischemia is Associated with Vascular Remodeling in Hippocampus. Kobe J Med Sci. 2018 Oct 15;64(3):E93-E106.  IHC-P ;  Rat.  
[IF=2.791] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156.  WB ;  Chicken.  
[IF=1.652] Wang Y et al. Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.(2019)Acta Histochem. Jan;121(1):56-63.  WB ;  Rat.  
[IF=0] Gao Y et al. Hepatoprotective effects of limb ischemic post-conditioning in hepatic ischemic rat model and liver cancer patients via PI3K/ERK pathways.(2018)Int J Biol Sci. Nov 3;14(14):2037-2050.  IHC ;  Rat.  
[IF=6.434] Yu D et al. Gestational exposure to acrylamide inhibits mouse placental development in vivo.(2018) J Hazard Mater.367:160-170.  WB ;  Mouse.  
[IF=3.208] Cui H et al. Gamma linolenic acid regulates PHD2 mediated hypoxia and mitochondrial apoptosis in DENinduced hepatocellular carcinoma. Drug Des Devel Ther. 2018 Dec 13;12:4241-4252.  WB ;  Rat.  
[IF=2.559] Jia C et al. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo(2018) Biochem Biophys Res Commun.505(3):720-725  WB ;  Mouse.  
[IF=4.679]   
[IF=4.087]   WB ;  Rat.  
[IF=3.063] Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245.  WB ;  broiler chick.  
[IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493.  WB&IHC ;  Rat.  
[IF=3.457] Chai FN et al. Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.Biomed Pharmacother. 2018 Jul;103:1002-1011.  WB ;  Mouse.  
[IF=4.686] Sun,et al.Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.(2018) Journal of Molecular Medicine. 96:281-299.  WB ;  Mouse.  
[IF=4.357] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137  WB ;  Human.  
[IF=1.173] Xu et al. Danshen attenuates cartilage injuries in osteoarthritis in vivo and in vitro by activating JAK2/STAT3 and AKT pathways. (2018) Exp.Anim. 67:127-137  WB ;  Rabbits.  
[IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057  WB ;  Human.  
[IF=4.26] Ma et al. A potential adjuvant chemotherapeutics, 18β-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. (2016) Sci.Re. 6:25396  WB ;  Human,Mouse.  
[IF=2.76] Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017).  WB ;  Rat.  
[IF=2.38] She, Yue, et al. "Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats." Cell Biochemistry and Biophysics (2015): 1-9.  IHC-P ;  Rat.  
[IF=2.27] He, X., et al. "Rapamycin inhibits acrolein‐induced apoptosis by alleviating ROS‐driven mitochondrial dysfunction in male germ cells." Cell Proliferation (2014).  WB ;  Mouse.  
[IF=1.922] Yong et al. Effects of compound 21, a non‑peptide angiotensin II type 2 receptor agonist, on general anesthesia‑induced cerebral injury in neonatal rats. (2018) Mol.Med.Rep. 18:5337-5344  WB ;  Rat.  
[IF=2.784] Zhang P et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. (2018) Int J Mol Med. 2018 Sep 19.  WB ;  Human.  
[IF=1.949] Li Q et al. The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway.(2018) Lasers Med Sci. Sep 15  WB ;  mouse.  
[IF=3.606] Xuejiao Zhou. et al. The novel ALK inhibitor ZX‐29 induces apoptosis through inhibiting ALK and inducing ROS‐mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02  WB ;  Human.  
[IF=4.432] Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585  WB ;  Human.  
[IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188  WB ;  Mouse.  
[IF=4.36] Yuhua Li. et al. Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. J Ethnopharmacol. 2022 Apr;287:114937  WB ;  Human.  
[IF=3.252] Ganggang Li. et al. Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells. Brain Res. 2022 Jan;1774:147723  WB ;  Rat.  
[IF=4.575] Ren, Teng-Teng. et al. Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation. Inflamm Res. 2021 Dec;70(10):1151-1164  WB ;  Mouse.  
[IF=3.636] Kaihui Xie. et al. ssc-microRNA-132 targets DACH1 to exert anti-inflammatory and anti-apoptotic effects in Clostridium perfringens beta2 toxin-treated porcine intestinal epithelial cells. Dev Comp Immunol. 2021 Sep;:104270  WB ;  pig.  
[IF=2.466] Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α. Tissue Cell. 2021 Sep;:101646  WB ;  rat.  
[IF=4.315] Huizhu Zhang. et al. Regulatory effects of HIF-1α and HO-1 in hypoxia-induced proliferation of pulmonary arterial smooth muscle cells in yak. Cell Signal. 2021 Nov;87:110140  WB ;  Bovine.  
[IF=7.086] Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625  WB ;  Mouse.  
[IF=2.952] Lu Ma. et al. Oxaliplatin promotes siMAD2L2‑induced apoptosis in colon cancer cells. Mol Med Rep. 2021 Sep;24(3):1-9  WB ;  Human.  
[IF=3.263] Tingting Wang. et al. A novel evodiamine amino derivative as a PI3K/AKT signaling pathway modulator that induces apoptosis in small cell lung cancer cells. Eur J Pharmacol. 2021 Sep;906:174215  WB ;  Human.  
[IF=3.105] Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68  WB ;  Rat.  
[IF=3.056] Shaofeng Wu. et al. Evaluation of toxic effects induced by arsenic trioxide or/and antimony on autophagy and apoptosis in testis of adult mice. 2021 May 20  WB ;  Mouse.  
[IF=6.304] Zhiqiang Han. et al. PNO1 regulates autophagy and apoptosis of hepatocellular carcinoma via the MAPK signaling pathway. Cell Death Dis. 2021 May;12(6):1-14  WB ;  Human.  
[IF=2.295] Shuman Zhang. et al. Crotonaldehyde exposure induces liver dysfunction and mitochondrial energy metabolism disorder in rats. 2021 Mar 22  WB ;  Rat.  
[IF=2.1] Xiangdong Meng. et al. Naringin ameliorates memory deficits and exerts neuroprotective effects in a mouse model of Alzheimer's disease by regulating multiple metabolic pathways. Mol Med Rep. 2021 May;23(5):1-13  WB ;  Mouse.  
[IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;:  WB ;  Rat.  
[IF=4.872] Yajing Wang. et al. Long-term exposure to the fluoride blocks the development of chondrocytes in the ducks: The molecular mechanism of fluoride regulating autophagy and apoptosis. Ecotox Environ Safe. 2021 Jul;217:112225  WB ;  Duck.  
[IF=1.785] Wen-Jun Gan. et al. Kuwanon G protects HT22 cells from advanced glycation end product-induced damage. Exp Ther Med. 2021 May;21(5):1-9  WB,IF ;  Mouse.  
[IF=4.556] Seung-Min Hong. et al. Intermittent Fasting Aggravates Lupus Nephritis through Increasing Survival and Autophagy of Antibody Secreting Cells in MRL/lpr Mice. Int J Mol Sci. 2020 Jan;21(22):8477  WB ;  Mouse.  
[IF=8.724] Yonggang Lv. et al. Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds. Bioact Mater. 2021 Sep;6:2767  WB ;  Human.  
[IF=4.087] Zhang Y et al. Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat modelPhytother Res.2020 Dec 10.  IHC ;  Rat.  
[IF=1.898] Chou P et al. Sodium Thiosulfate Ameliorates Renovascular Hypertension-Induced Renal Dysfunction and Injury in RatsKidney Blood Press Res.2020 Dec 16;1-16.  WB ;  Rat.  
[IF=2.997] Yalei Yuet al. Cantharidin‐induced acute hepatotoxicity: the role of TNF‐α, IKK‐α, Bcl‐2, Bax and caspase3. J Appl Toxicol . 2020 Nov;40(11):1526-1533.  WB ;  rat.  
[IF=3.895] Li P et al. Selenium Yeast Alleviates Ochratoxin A-Induced Hepatotoxicity via Modulation of the PI3K/AKT and Nrf2/Keap1 Signaling Pathways in Chickens. Toxins (Basel). 2020 Feb 25;12(3).  WB ;  chicken.  
[IF=6.551] Wei J et al. Endosulfan induces cardiotoxicity through apoptosis via unbalance of pro-survival and mitochondrial-mediated apoptotic pathways. Sci Total Environ . 2020 Jul 20;727:138790.  WB ;  human.  
[IF=4.527] Wan F et al. Long-term exposure to copper induces autophagy and apoptosis through oxidative stress in rat kidneys. Ecotoxicol Environ Saf. 2020 Mar 1;190:110158.  WB ;  Rat.  
[IF=3.329] Zhang Y et al. Overexpressed CES2 has prognostic value in CRC and knockdown CES2 reverses L-OHP-resistance in CRC cells by inhibition of the PI3K signaling pathway. Exp Cell Res. 2020 Jan 22:111856.  WB ;  Human.  
[IF=3.499] Zhan C et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2019 Oct 1. pii: S0378-4274(19)30295-4.  IHC-P ;  Rat.  
[IF=2.929] Yu Y et al. Exosomal miR-199a-5p derived from endothelial cells attenuates apoptosis and inflammation in neural cells by inhibiting endoplasmic reticulum stress. Brain Res. 2019 Oct 18:146515.  WB ;  Human.  
[IF=8.063] Luo J et al. ATF4 destabilizes RET through nonclassical GRP78 inhibition to enhance chemosensitivity to bortezomib in human osteosarcoma. Theranostics. 2019 Aug 14;9(21):6334-6353.  IHC-P ;  Human.  
[IF=4.784] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9.  IHF ;  Rat.  
[IF=3.571] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149.  WB&IHC-P ;  Rat.  
[IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577  WB ;  
[IF=2.634] Guo MB et al. Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells.Eur Spine J. 2018 Oct;27(10):2609-2620.  WB ;  Rabbit.  
[IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316.  WB ;  Chicken.  
[IF=3.616] Geng X et al. Role of ZIP8 in regulation of cisplatin sensitivity through Bcl-2. (2018) Toxicol. Appl. Pharmacol. Oct 17;362.  IF ;  Mouse.  
[IF=2.341] Ling,et al.Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial‑mesenchymal transition in diabetic rats.(2018) International Journal of Molecular Medicine. 41:2704-2714.  IHC-P ;  Rat.  
[IF=2.561] Peng X et al. Overexpression of modified human TRβ1 suppresses the growth of hepatocarcinoma SK-hep1 cells in vitro and in xenograft models.Mol Cell Biochem. 2018 Dec;449(1-2):207-218.  IHC-P&WB ;  Mouse&Human.  
[IF=0] Zhou et al. Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. (2012) Cancers.(Basel). 4:1318-32  WB ;  Human.  
[IF=1.894] Chi et al. Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model. (2018) Med.Sci.Monit. 24:3946-3957  WB ;  Rat.  
[IF=0] Zhang et al. Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice. (2014) Evid.Based.Complement.Alternat.Me. 2013:690808  IHC-P ;  Mouse.  
[IF=2.85] Li et al. A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. (2017) PLoS.On. 12:e0175407  WB ;  Human.  
[IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458  WB ;  Human.  
[IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).  WB ;  Human.  
[IF=3.33] Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.  WB ;  Human.  
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.  
[IF=3.33] Tian, Xiao Qiang, et al. "The protective effect of Hyperbaric oxygen and Ginkgo Biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats." Behavioural Brain Research (2012).  WB ;  Rat.  
研究领域 细胞生物  信号转导  细胞凋亡  细胞类型标志物  肿瘤细胞生物标志物  新陈代谢  线粒体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 26kDa
检测分子量 26 kDa
细胞定位 细胞核 细胞浆 细胞膜 线粒体
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Bcl-2: 101-160/236 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.

Function:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Subunit:
Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex.

Subcellular Location:
Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Tissue Specificity:
Expressed in a variety of tissues.

Post-translational modifications:
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.

DISEASE:
Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

Similarity:
Belongs to the Bcl-2 family.

SWISS:
P10415

Gene ID:
596

Database links:

Entrez Gene: 281020 Cow

Entrez Gene: 596 Human

Entrez Gene: 12043 Mouse

Entrez Gene: 24224 Rat

Omim: 151430 Human

SwissProt: O02718 Cow

SwissProt: P10415 Human

SwissProt: P10417 Mouse

SwissProt: P49950 Rat

Unigene: 150749 Human

Unigene: 257460 Mouse

Unigene: 9996 Rat



Bcl-2基因是指B-cell lymphoma gene。人体滤泡B细胞淋巴瘤中过量表达的原癌基因。由于染色体t(14;18)易位,将Bcl-2基因置于免疫球蛋白重链的转录调控下,使其表达失控。在细胞系中其过量表达能延长细胞存活期而不诱导细胞增殖。它是哺乳动物中细胞调亡的抑制基因。参与细胞凋亡的调控。肿瘤中的Bcl-2基因可提高侵润性瘤细胞的生存能力。主要用于滤胞型淋巴瘤、毛细管性白血病及细胞凋亡等方面的研究。

目前研究认为:Bcl-2也是细胞凋亡的一种抑制因子、参与细胞凋亡调控,可以用于各种恶性肿瘤的细胞凋亡的研究。
产品图片
Protein: Spleen(Mouse)lysate 30ug;
Primary: Anti-Bcl-2(bs-0032R) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size : 26kD
Observed band size : 26kD
Protein: Brain(Mouse)lysate 30ug;
Primary: Anti-Bcl-2(bs-0032R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size : 26kD
Observed band size : 26kD
Sample:
Bcl-2 overexpression E.coli lysate (whole cell) Lysate at 40 ug
Recombinant protein Lysate at 40 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 25 kD
Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Sample:
MCF-7 (Human) Cell Lysate at 30 ug
Hela (Human) Cell Lysate at 30 ug
HL60 (Human) Cell Lysate at 30 ug
A431 (Human) Cell Lysate at 30 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 23 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
RAW264.7 Cell (Mouse) Lysate at 40 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-0032R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B cell lymphoma 2; Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes and DAPI for nuclei staining.
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