产品中心-北京博奥森生物技术有限公司

产品编号	bs-0466R
英文名称	Beta casein Rabbit pAb
中文名称	β-酪蛋白抗体
别名	beta-casein; CASB; CASB_SHEEP; Casein beta; CSN 2; Csn2.
	Specific References (14) \| bs-0466R has been referenced in 14 publications. [IF=6.117] Gang Lin. et al. ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. J NUTR BIOCHEM. 2023 Jan;:109274 IF ; Mouse. PubMed:36681308 [IF=6.048] Hao Qi. et al. Methionine and leucine induce ARID1A degradation to promote mTOR expression and milk synthesis in mammary epithelial cells. J Nutr Biochem. 2021 Nov;:108924 WB,IF ; Cow. PubMed:34843932 [IF=5.895] Xinling Wang. et al. Enhancement of BCAT2-Mediated Valine Catabolism Stimulates β-Casein Synthesis via the AMPK-mTOR Signaling Axis in Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX WB, IF ; Bovine. PubMed:35916279 [IF=5.561] Xinyang Fan. et al. CEBPA-Regulated Expression of SOCS1 Suppresses Milk Protein Synthesis through mTOR and JAK2-STAT5 Signaling Pathways in Buffalo Mammary Epithelial Cells. FOODS. 2023 Jan;12(4):708 WB ; Bovine. PubMed:36832783 [IF=5.396] Zhiyun Hao. et al. MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells. Food Funct. 2021 Aug;: WB ; Sheep. PubMed:10.1039/D1FO01260F [IF=5.223] Xinyang Fan. et al. MiR-190a regulates milk protein biosynthesis through the mTOR and JAK2–STAT5 signaling pathways by targeting PTHLH in buffalo mammary epithelial cells. J FUNCT FOODS. 2023 Mar;102:105451 WB ; Bovine. PubMed:10.1016/j.jff.2023.105451 [IF=5.168] Xinling Wang. et al. Plasma Proteomic Profiling Reveals the Regulatory Factors of Milk Protein Synthesis in Holstein Cows. BIOLOGY-BASEL. 2022 Aug;11(8):1239 WB ; Bovine. PubMed:36009866 [IF=4.092] Ludmila K et al. Development of Immunohistochemical Methods for Casein Detection in Meat ProductsFoods.2020 Dec 24;10(1):28. IHC ; Cow、Pig. PubMed:33374134 [IF=4.074] Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res . 2020 Aug 7;19(8):3211-3219. WB ; Cow. PubMed:32506918 [IF=3.571] Li P et al. CRTC2 is a key mediator of amino acid-induced milk fat synthesis in mammary epithelial cells. J Agric Food Chem. 2019 Sep 18;67(37):10513-10520. WB ; Cow. PubMed:31475823 [IF=3.333] Jinyu Zhou et al. Prolactin regulates LAT1 expression via STAT5 (signal transducer and activator of transcription 5) signaling in mammary epithelial cells of dairy cows. J Dairy Sci. 2020 Jul;103(7):6627-6634. WB ; Cow. PubMed:32389479 [IF=1.778] Z.Y. Hao. et al. Deep small RNA-Seq reveals microRNAs expression profiles in lactating mammary gland of 2 sheep breeds with different milk performance. Domest Anim Endocrin. 2021 Jan;74:106561 IHC ; Sheep. PubMed:33035848 [IF=1.421] Li B et al. Septin6 regulates cell growth and casein synthesis in dairy cow mammary epithelial cells viamTORC1 pathway. J Dairy Res. 2019 May;86(2):181-187. WB ; Dairy cow. PubMed:31122298 [IF=] IHC ; ewes. PubMed:33035848
研究领域	细胞生物免疫学
抗体来源	Rabbit
克隆类型	Polyclonal
克隆号
交叉反应	Human,Cow,Goat (predicted: Sheep)
产品应用	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	23 kDa
检测分子量
细胞定位	分泌型蛋白
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from sheep Beta-casein: 151-222/222
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Milk proteins are crucial for the development of all newborn mammals and caseins constitute the major proteins in mammalian milk. b- and k-caseins are the only caseins present in human milk. The b-casein/k-casein ratio is higher in colostrum than in transitional and mature milk and is related to a better digestibility of colostrum casein micelles by the neonate during the first days of life. Human b-casein-encoding gene (Bca) contains a highly phosphorylated site, which is responsible for the calcium-binding capacity of b-casein. A common set of transcription factors are required for the expression of b-casein. Multiple binding sites for Stat5, C/EBPb (CCAAT/enchancer-binding protein) and several half-sites for glucocorticoid receptor (GR) are identified in the distal human enhancer of the b-casein gene. b-casein gene transcription is regulated primarily by a composite response element (CoRE), which integrates signaling from the lactogenic hormones PRL, insulin and hydrocortisone in mammary epithelial cells. NFkB functions as a negative regulator of b-casein gene expression during pregnancy by interfering with Stat5 tyrosine phosphorylation. Function: Important role in determination of the surface properties of the casein micelles. Subcellular Location: Secreted. Tissue Specificity: Mammary gland specific. Secreted in milk. Similarity: Belongs to the beta-casein family. Gene ID: 443391 Database links: Entrez Gene: 443391 Sheep Entrez Gene: 1447 Human Omim: 115460 Human SwissProt: P05814 Human Unigene: 2242 Human β-酪蛋白常在乳腺表达，山羊、绵羊同源分子量：25kDa
产品图片	Sample: Lane 1: Milk (Goat) Lysate at 2 ug Lane 2: Milk (Cow) Lysate at 30 ug Lane 3: Milk (Cow) Lysate at 3 ug Lane 4: Milk (Human) Lysate at 30 ug Primary: Anti-Beta-casein (bs-0466R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 25-29 kD

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记