产品编号 | bs-0060R |
英文名称 | CEA |
中文名称 | 癌胚抗原抗体 |
别 名 | Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100. |
Specific References (4) | bs-0060R has been referenced in 4 publications.
[IF=6.057] Limin Zhou. et al. Copper doped terbium metal organic framework as emitter for sensitive electrochemiluminescence detection of CYFRA 21-1. Talanta. 2022 Feb;238:123047
[IF=2.678] Li J et al. A highly specific and sensitive fluorescence quenching probe for carcinoembryonic antigen detection based on tetrapod Au nanostars with Ag coating. Materials Today Communications (2020). Other ;
[IF=2.678] Jian-Jun Liet al. A highly specific and sensitive fluorescence quenching probe for carcinoembryonic antigen detection based on tetrapod Au nanostars with Ag coating. Materials Today Communications, 101373. other ;
[IF=0] Chen L et al. Local extraction and detection of early stage breast cancers through a microneedle and nano-Ag/MBL film based painless and blood-free strategy. Materials Science and Engineering: C,2019. 110402. Other ;
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研究领域 | 肿瘤 免疫学 干细胞 肿瘤细胞生物标志物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 71kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CEA: 51-120/349 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Subunit: Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Post-translational modifications: Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. Similarity: Belongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains. SWISS: P40199 Gene ID: 4680 Database links: Entrez Gene: 4680 Human Omim: 163980 Human SwissProt: P40199 Human Unigene: 466814 Human Unigene: 122781 Rat CEA是一种胚胎性抗原,主要存在于胎儿消化道上皮组织,胰腺和肝癌中。CEA广泛存在各种上皮性肿瘤,尤其是胃肠道恶性肿瘤,CEA分布阳性类型与肿瘤的恶性度有关。CEA与EMA一样可作为上皮性恶性肿瘤的重要标记。 |
产品图片 |
Sample: SW480 Cell Lysate at 40 ug
Primary: Anti- CEA(bs-0060R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 71 kD Observed band size: 71 kD
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADRAIA) Polyclonal Antibody, Unconjugated (bs-0060R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CEA/CD66e/CEACAM5 Polyclonal Antibody, Unconjugated(bs-0060R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-CEA antibody (bs-0060R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol overnight at 4℃ . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |