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Rabbit Anti-CNPase  antibody (bs-1000R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1000R
英文名称 CNPase
中文名称 2’,3’-环腺苷酸-3’-磷酸二酯酶抗体
别    名 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNPase; Cnp; Cnp-1; Cnp1; 2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 2'3'-cyclic-nucleotide 3'-phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1.  
Specific References  (3)     |     bs-1000R has been referenced in 3 publications.
[IF=2.889] Bin Liu. et al. Nicotinamide Improves Cognitive Function in Mice With Chronic Cerebral Hypoperfusion. Front Neurol. 2021; 12: 596641  WB,IHC ;  Mouse.  
[IF=2.882] Gul Fatma Yarim. et al. Nobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model. Fitoterapia. 2022 Jan;156:105099  IHC ;  Rat.  
[IF=2.43] Li, Ping, et al. "CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan." World Journal of Gastroenterology: WJG 21.5 (2015): 1518.  IHC-P ;  Rat.  
研究领域 神经生物学  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Cow,Horse,Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 48kDa
细胞定位 细胞浆 细胞外基质 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CNPase: 151-250/421 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. [provided by Alliance of Genome Resources, Nov 2021]

Function:
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.

Subunit:
Exists as monomers and homodimers.

Subcellular Location:
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the cyclic nucleotide phosphodiesterase family.

SWISS:
P09543

Gene ID:
1267

Database links:

Entrez Gene: 280752 Cow

Entrez Gene: 607694 Dog

Entrez Gene: 1267 Human

Entrez Gene: 12799 Mouse

Entrez Gene: 493772 Pig

Entrez Gene: 25275 Rat

Omim: 123830 Human

SwissProt: P06623 Cow

SwissProt: P09543 Human

SwissProt: P16330 Mouse

SwissProt: P18104 Pig

SwissProt: P13233 Rat

SwissProt: P56283 Sheep

Unigene: 273621 Human

Unigene: 15711 Mouse

Unigene: 31762 Rat



产品图片
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-CNPase(bs-1000R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 46 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-CNPase (bs-1000R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 48kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNPase) Polyclonal Antibody, Unconjugated (bs-1000R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CNP/CNPase Polyclonal Antibody, Unconjugated(bs-1000R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CNP/CNPase Polyclonal Antibody, Unconjugated(bs-1000R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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