产品中心-北京博奥森生物技术有限公司

产品编号	bs-0470R
英文名称	Osteocalcin
中文名称	骨钙蛋白/骨钙素抗体
别名	Osteocalcin Propeptide; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN.
	Specific References (31) \| bs-0470R has been referenced in 31 publications. [IF=16.744] Jianxu Wei. et al. Switch-on mode of bioenergetic channels regulated by curcumin-loaded 3D composite scaffold to steer bone regeneration. CHEM ENG J. 2023 Jan;452:139165 IHC ; Rat. PubMed:10.1016/j.cej.2022.139165 [IF=15.304] Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683 IHC ; Rat. PubMed:35870263 [IF=12.822] Lan Li. et al. Design of a Haversian system-like gradient porous scaffold based on triply periodic minimal surfaces for promoting bone regeneration. J ADV RES. 2023 Jan;: WB ; Mouse. PubMed:36632888 [IF=11.322] Zhenyin Chen. et al. Gelatin/sodium alginate composite hydrogel with dynamic matrix stiffening ability for bone regeneration. COMPOS PART B-ENG. 2022 Aug;243:110162 IHC ; Rat. PubMed:10.1016/j.compositesb.2022.110162 [IF=11.092] Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047 IHC ; Rat. PubMed:37059691 [IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039 IF,IHC ; Mouse. PubMed:33511306 [IF=8.097] Hu Q et al. Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration. ACS Appl Mater Interfaces. 2018 Aug 22;10(33):27669-27680. IHC-P ; Rabbit. PubMed:30063134 [IF=7.655] Liu Jincheng. et al. WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis. Stem Cells Translational Medicine. 2023 Apr;: WB ; Mouse. PubMed:37010483 [IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359 IHC ; Rat. PubMed:34474906 [IF=7.242] Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;: IF ; Mouse. PubMed:33711525 [IF=6.832] Hou, Yonghui. et al. Nonwoven-based gelatin/polycaprolactone membrane loaded with ERK inhibitor U0126 for treatment of tendon defects. Stem Cell Res Ther. 2022 Dec;13(1):1-11 IHC ; Rat. PubMed:35012661 [IF=5.923] Sachin Chaugule. et al. Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling. Int J Mol Sci. 2021 Jan;22(19):10289 IF ; mouse. PubMed:34638628 [IF=5.344] Ling-Kun Zhang. et al. Growth factors regional patterned and photoimmobilized scaffold applied to bone tissue regeneration. J Mater Chem B. 2020 Dec;8(48):10990-11000 IHC ; Rat. PubMed:33300520 [IF=4.85] Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549 IHC ; Mouse. PubMed:36464103 [IF=3.768] Ohsugi Y et al. Evaluation of bone healing following Er: YAG laser ablation in rat calvaria compared with bur drilling.(2018)J Biophotonics.Oct 15:e201800245. IHC ; Rat. PubMed:30324711 [IF=3.68] Zhang, Ping, et al. WB ; Rat. PubMed:22237862 [IF=3.496] Liu Y et al. Fabrication of Biomolecule-Loaded Composite Scaffolds Carried by Extracellular Matrix Hydrogel IF ; Human. PubMed:33023406 [IF=3.485] Bai J et al. Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. Am J Physiol Cell Physiol . 2020 May 1;318(5):C1005-C1017. FCM ; rat. PubMed:32233952 [IF=3.373] Quade M et al. Strontium enhances BMP-2 mediated bone regeneration in a femoral murine bone defect model. J Biomed Mater Res B Appl Biomater. 2019 Apr 5. ICC ; Mouse. PubMed:30950569 [IF=3.331] Ning T et al. Aging affects the proliferation and mineralization of rat dental pulp stem cells under inflammatory conditions. Int Endod J. 2019 Aug 16. WB&IHC-P ; Rat. PubMed:31419325 [IF=3.234] Fan S et al. Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway.Life Sci. 2018 Aug 15;207:205-211. WB ; Rat. PubMed:29883721 [IF=3.144] Li Jianbin. et al. Characterizations of alveolar repair after mandibular second molar extraction: an experimental study in rats. J APPL ORAL SCI. 2022 Jul;30: IHC ; Rat. PubMed:35830122 [IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017). WB ; Human. PubMed:28608628 [IF=2.985] Jiating Lin. et al. Metformin promotes the osseointegration of titanium implants under osteoporotic conditions by regulating BMSCs autophagy, and osteogenic differentiation. Biochem Bioph Res Co. 2020 Oct;531:228 WB ; Rat. PubMed:32800340 [IF=2.885] Sheng, Zhenxian. et al. Denervation delays initial bone healing of rat tooth extraction socket. ODONTOLOGY. 2022 Dec;:1-9 IF ; Rat. PubMed:36512167 [IF=2.639] Jiarong Yanget al. Effects of Different Doses of Calcium on the Mitochondrial Apoptotic Pathway and Rho/ROCK Signaling Pathway in the Bone of Fluorosis Rats. Biol Trace Elem Res . 2020 Jul 25. IHC ; rat. PubMed:32710350 [IF=2.633] Jingzhi Li. et al. TGF-β2 and TGF-β1 differentially regulate the odontogenic and osteogenic differentiation of mesenchymal stem cells. Arch Oral Biol. 2022 Mar;135:105357 WB ; Human. PubMed:35085927 [IF=2.304] Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med . 2020 Aug;181:105049. WB ; Hen. PubMed:32526547 [IF=1.931] Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034 WB ; Human. PubMed:33472098 [IF=1.476] Kanata IBI. et al. Spontaneous extraskeletal osteosarcoma in the neck skeletal muscle of a Crlj:CD1 (ICR) mouse. 2021 Jan 18 IHC ; Rat, Mouse. PubMed:10.1293/tox.2020-0083
研究领域	免疫学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse (predicted: Rat,Dog,Cow,Horse)
产品应用	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	11kDa
细胞定位	分泌型蛋白
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human BGP: 21-80/100
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Subcellular Location: Secreted. Post-translational modifications: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. Similarity: Belongs to the osteocalcin/matrix Gla protein family. Contains 1 Gla (gamma-carboxy-glutamate) domain. SWISS: P02818 Gene ID: 632 Database links: Entrez Gene: 281646 Cow Entrez Gene: 632 Human Entrez Gene: 397530 Pig Entrez Gene: 25295 Rat Omim: 112260 Human SwissProt: P02820 Cow SwissProt: P02818 Human SwissProt: Q8HYY9 Pig SwissProt: P04640 Rat Unigene: 654541 Human Unigene: 9722 Rat 骨钙素又称骨γ-羧谷氨酸包含蛋白
产品图片	Tissue/cell: bone of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Osteocalcin Polyclonal Antibody, Unconjugated(bs-0470R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (bs-0470R) at 1:100 followed by conjugation to the secondary antibody and DAB staining

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记