产品中心-北京博奥森生物技术有限公司

产品编号	bs-1012R
英文名称	BMP2
中文名称	骨形态发生蛋白2抗体
别名	Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN.
	Specific References (39) \| bs-1012R has been referenced in 39 publications. [IF=15.153] Haojun Liang. et al. A Bone-Penetrating Precise Controllable Drug Release System Enables Localized Treatment of Osteoporotic Fracture Prevention via Modulating Osteoblast-Osteoclast Communication. SMALL. 2023 Mar;:2207195 IHC ; Rat. PubMed:36971278 [IF=11.322] Chengzhong Xu. et al. Microenvironment responsive hypoxia-mimetic DFO composite hydrogel for on-demand neovascularization to promote tendon-to-bone healing. COMPOS PART B-ENG. 2023 Apr;:110726 IF ; Human,Rat,Mouse. PubMed:10.1016/j.compositesb.2023.110726 [IF=10.652] Zhiwei Jiangaet al. An effective light activated TiO2 nanodot platform for gene delivery within cell sheets to enhance osseointegration. WB ; Human. PubMed:10.1016/j.cej.2020.126170 [IF=9.417] Bangbang Liang. et al. Proportion-dependent osteogenic activity of electrospun nano-hydroxyapatite/polylactic acid fiber membrane in vitro and in vivo. MATER DESIGN. 2022 Jul;219:110834 WB ; Rat. PubMed:10.1016/j.matdes.2022.110834 [IF=8.38] Naeem, A. S., et al. "AKT1-mediated Lamin A/C degradation is required for nuclear degradation and normal epidermal terminal differentiation." Cell Death & Differentiation (2015). WB ; Rat. PubMed:26045045 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC ; Rat. PubMed:10.1016/j.apmt.2020.100673 [IF=8.071] Yifan Pan. et al. Disturbance in transcriptomic profile, proliferation and multipotency in human mesenchymal stem cells caused by hexafluoropropylene oxides. Environ Pollut. 2022 Jan;292:118483 IF ; Human. PubMed:34763017 [IF=6.064] Yu Hedong. et al. Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs. FRONT BIOENG BIOTECH. 2022 Aug;0:1275 WB ; Rabbit. PubMed:35992335 [IF=5.58] Zhang, Jing, et al. "Combination of simvastatin, calcium silicate/gypsum, and gelatin and bone regeneration in rabbit calvarial defects." Scientific Reports 6 (2016): 23422. IHC-P ; Rabbit. PubMed:26996657 [IF=5.546] Taiga Ono. et al. Generation of biohybrid implants using a multipotent human periodontal ligament cell line and bioactive core materials. 2021 Feb 19 IF ; Human. PubMed:33604904 [IF=5.395] Yuanchao Zhu. et al. Biomimetic Porous Magnesium Alloy Scaffolds Promote the Repair of Osteoporotic Bone Defects in Rats through Activating the Wnt/β-Catenin Signaling Pathway. ACS BIOMATER-SCI ENG. 2023;XXXX(XXX):XXX-XXX IHC ; Rat. PubMed:37200162 [IF=5.08] Hsieh MK et al. BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model.Mater Sci Eng C Mater Biol Appl. 2018 Oct 1;91:806-816. IHC-P ; Rat. PubMed:30033316 [IF=4.225] Shiyi Huang. et al. Transforming Growth Factor-β3/Recombinant Human-like Collagen/Chitosan Freeze-Dried Sponge Primed With Human Periodontal Ligament Stem Cells Promotes Bone Regeneration in Calvarial Defect Rats. Front Pharmacol. 2021; 12: 678322 IHC ; Rat. PubMed:33967817 [IF=4.101] Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13 WB ; Sheep. PubMed:34132357 [IF=3.934] Li Zhilang. et al. Uterine Scarring Leads to Adverse Pregnant Consequences by Impairing the Endometrium Response to Steroids. Endocrinology. 2020 Nov;161(11): PubMed:32976565 [IF=3.616] Lv N et al. The roles of bone morphogenetic protein 2 in perfluorooctanoic acid induced developmental cardiotoxicity and l-carnitine mediated protection.Toxicol Appl Pharmacol. 2018 Aug 1;352:68-76. WB&IHC ; Chicken. PubMed:29800642 [IF=3.573] Liu, Jie. et al. Phosphorylated PAMAM dendrimers: an analog of dentin non-collagenous proteins, enhancing the osteo/odontogenic differentiation of dental pulp stem cells. 2021 Sep 13 WB ; human. PubMed:34515858 [IF=3.378] Song, Yanqiu. et al. Iron overload impairs renal function and is associated with vascular calcification in rat aorta. BIOMETALS. 2022 Sep;:1-15 WB ; Rat. PubMed:36178540 [IF=3.375] Gao R et al. Three-dimensional-printed titanium alloy porous scaffold combined with trans-cinnamaldehyde for repairing osteonecrosis of the femoral head in a dog model. Am J Transl Res . 2020 Mar 15;12(3):1070-1079. IHC ; beagle dogs. PubMed:32269735 [IF=3.246] Shimin Hao. et al. Evaluation of Biodegradable Alloy Fe30Mn0.6N in Rabbit Femur and Cartilage through Detecting Osteogenesis and Autophagy. BIOMED RES INT. 2023 Jan 18;2023:3626776 IHC ; Rabbit. PubMed:36714031 [IF=3.161] Yılmaz, Birtan Tolga. et al. In vivo efficacy of low-level laser therapy on bone regeneration. Laser Med Sci. 2022 Jan;:1-8 IHC ; Rabbit. PubMed:35022870 [IF=2.86] Kishi, Yuta, et al. "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway."International Journal of Molecular Sciences 16.10 (2015): 24820-24838. WB ; Mouse. PubMed:26492236 [IF=2.784] Chen X et al. Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Send to Int J Mol Med. 2019 Apr 24. WB ; Human. PubMed:31017269 [IF=2.768] Li Y et al. Sulfonated chitosan oligosaccharide alleviates the inhibitory effect of basic fibroblast growth factor on osteogenic differentiation of human periodontal ligament stem cells. J Periodontol. 2019 Oct 1. WB ; Human. PubMed:31573683 [IF=2.65] Kai Wang. et al. Network Pharmacological Analysis and Animal Experimental Study on Osteoporosis Treatment with GuBen-ZengGu Granules. EVID-BASED COMPL ALT. 2023 Jan 12;2023:9317557 WB ; Rat. PubMed:36686973 [IF=2.639] Jiarong Yanget al. Effects of Different Doses of Calcium on the Mitochondrial Apoptotic Pathway and Rho/ROCK Signaling Pathway in the Bone of Fluorosis Rats. Biol Trace Elem Res . 2020 Jul 25. IHC ; rat. PubMed:32710350 [IF=2.38] Zhu, Wenjing, et al. "Effect of PI3K/Akt Signaling Pathway on the Process of Prostate Cancer Metastasis to Bone." Cell Biochemistry and Biophysics (2015): 1-7. WB ; Human. PubMed:22870197 [IF=2.341] Diao S et al. IGF2 enhanced the osteo‐/dentinogenic and neurogenic differentiation potentials of stem cells from apical papilla. J Oral Rehabil. 2019 Jul 10. WB ; Human. PubMed:31291686 [IF=2.31] Wei B et al. Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits. J Bone Miner Metab. 2020 Jan 24. ELISA ; Rabbit. PubMed:31980897 [IF=2.24] Wu, Mei, et al. "Expression analysis of BMP2, BMP5, BMP10 in human colon tissues from Hirschsprung disease patients." Int J Clin Exp Pathol 7.2 (2014): 529-536. IHC-P,WB ; Human. PubMed:24551273
研究领域	细胞生物免疫学发育生物学神经生物学生长因子和激素
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Chicken,Dog,Cow,Horse,Rabbit,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	13/44kDa
细胞定位	分泌型蛋白
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human BMP2: 283-365/396
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis. Function: Induces cartilage and bone formation. Subunit: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN. Subcellular Location: Secreted. Tissue Specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. Similarity: Belongs to the TGF-beta family. SWISS: P12643 Gene ID: 650 Database links: Entrez Gene: 650 Human Entrez Gene: 12156 Mouse Entrez Gene: 29373 Rat Omim: 112261 Human SwissProt: P12643 Human SwissProt: P21274 Mouse SwissProt: P49001 Rat Unigene: 73853 Human Unigene: 103205 Mouse Unigene: 90931 Rat 神经干细胞标志物 BMP-2主要对未分化间充质细胞和骨系细胞起到募集和分化作用。在骨形成早期，BMP-2不仅可使未分化间质细胞向骨形成中心募集，并分化为骨系细胞，而且可使成纤维细胞、成肌细胞及骨髓的基细胞逆转分化为骨系细胞。其主要过程是：增加或抑制这些细胞内的某些特异性蛋白的分泌，使成纤维细胞分化为成骨细胞，成肌细胞快速分化为肥大的软骨细胞，并促进基质钙化。对于成骨细胞，BMP-2则可使之维持其特有细胞表型，并诱导成骨细胞标志物的增高，促进细胞外基质钙化。在骨形成后期，BMP-2还作为一种破骨细胞分化因子与其它支持破骨细胞分化因子直接或间接刺激破骨细胞分化，参与骨的重建。
产品图片	Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- BMP2 (bs-1012R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 13 kD Observed band size: 11 kD Sample: Lane 1: U2os (Human) Cell Lysate at 30 ug Lane 2: MG63 (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Primary: Anti-BMP2 (bs-1012R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60/45 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP2) Polyclonal Antibody, Unconjugated (bs-1012R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated(bs-1012R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记