扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-CD272  antibody (bs-0624R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0624R
英文名称 CD272
中文名称 B和T淋巴细胞衰减蛋白抗体
别    名 B and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; BTLA; BTLA1; CD272 antigen; FLJ16065; MGC129743; BTLA_HUMAN; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein.  
研究领域 肿瘤  细胞生物  免疫学  淋巴细胞  t-淋巴细胞  b-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 28kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human B and T-lymphocyte attenuator: 221-289/289 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 B and T lymphocyte attenuator (BTLA), an immunoglobulin domain-containing glycoprotein with two immunoreceptor tyrosine-based inhibitory motifs. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. Crosslinking BTLA with antigen receptors induces its tyrosine phosphorylation and association with the Src homology domain 2 (SH2)-containing protein tyrosine phosphatases SHP-1 and SHP-2, and attenuates production of interleukin 2 (IL-2). BTLA-deficient T cells show increased proliferation, and BTLA-deficient mice have increased specific antibody responses and enhanced sensitivity to experimental autoimmune encephalomyelitis. B7x, a peripheral homolog of B7, is a ligand of BTLA. Thus, BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).

Function:
Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.

Subunit:
Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Interacts with TNFRSF14/HVEM.

Subcellular Location:
Membrane; Single-pass type I membrane protein (Potential).

Post-translational modifications:
Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
N-glycosylated.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q7Z6A9

Gene ID:
151888

Database links:

Entrez Gene: 151888 Human

Entrez Gene: 208154 Mouse

Entrez Gene: 407756 Rat

Omim: 607925 Human

SwissProt: Q7Z6A9 Human

SwissProt: Q7TSA3 Mouse

SwissProt: Q6PNM1 Rat

Unigene: 445162 Human

Unigene: 38199 Mouse

Unigene: 124474 Rat



BTLA对T细胞的活化、增殖起着重要的负调控作用,BTLA相应配体为TNFR超家族中的疱疹病毒入侵介质(HVEM), 其表达于包括T细胞在内的多种免疫细胞表面。
有学者将他定为CD28的超级族成员,B、T淋巴细胞衰减子-CD272主要用于细胞信号传导方面的研究。
近来国外学者对BTLA用于抑制肿瘤方面的研究也有了新的进展,认为B、T淋巴细胞衰减子对肿瘤的生长有抑制作用,探索新的肿瘤免疫治疗有了新的途径, 封闭此途径有可能成为肿瘤免疫治疗的新靶点。
产品图片
Sample:
Lane 1: Rat Thymus tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-CD272 (bs-0624R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 55 kDa
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CD272/BTLA Polyclonal Antibody, Unconjugated(bs-0624R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
版权所有 2004-2026 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    认证编号: 00122Q31509R1M/1100
京ICP备05066980号-1         京公网安备110107000727号