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Rabbit Anti-CD44  antibody (bs-0521R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0521R
英文名称 CD44
中文名称 CD44抗体
别    名 LHR; BA-1; CD 44; CD44 antigen; CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; Hyaluronate receptor; MDU2; MDU3; MIC4; MUTCH1; PGP-1; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; CD44 antigen isoform 1 precursor.  
Specific References  (17)     |     bs-0521R has been referenced in 17 publications.
[IF=25.841] Xin Zhou. et al. Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation. J Extracell Vesicles. 2022 Mar;11(3):e12198  WB ;  Human.  
[IF=17.521] Qing Li. et al. SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3β/β-Catenin Signaling. Advanced Science. 2023 Feb;:2203573  WB ;  Mouse.  
[IF=10.317]   WB ;  human.  
[IF=10.317] Kaiyuan Wanget al. An exosome-like programmable-bioactivating paclitaxel prodrug nanoplatform for enhanced breast cancer metastasis inhibition. Biomaterials . 2020 Oct;257:120224.  WB ;  Human.  
[IF=8.352] Chanjuan Dong. et al. Graphene-based conductive fibrous scaffold boosts sciatic nerve regeneration and functional recovery upon electrical stimulation. Appl Mater Today. 2020 Dec;21:100870  IHC ;  Rat.  
[IF=7.727] Xue Yanget al. “Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer. J Control Release . 2020 Oct 10;326:615-627.  WB ;  Human.  
[IF=5.1] Neal et al. The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor. (2018) J.Neuroinflammation. 15:73  IF(ICC) ;  Mouse.  
[IF=4.3] Xiong et al. Bone Marrow Mesenchymal Stem-Cell Transplantation Promotes Functional Improvement Associated with CNTF-STAT3 Activation after Hemi-Sectioned Spinal Cord Injury in Tree Shrews. (2017) Front.Cell.Neurosci. 11:172  tree shrews.  
[IF=4.175] Yue Wu. et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int. 2021 Dec;21(1):1-14  FC ;  Human.  
[IF=3.647] Xiaohui Chen. et al. Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-497-5p inhibit RSPO2 and accelerate OPLL. Life Sci. 2021 Apr;:119481  FC ;  Human.  
[IF=2.626] Zhao, Gang. et al. LINC02381, a sponge of miR-21, weakens osteogenic differentiation of hUC-MSCs through KLF12-mediated Wnt4 transcriptional repression. 2021 Nov 15  WB ;  Mouse,Human.  
[IF=1.69] Xu et al. NIBP impacts on the expression of E-cadherin, CD44 and vimentin in colon cancer via the NF-κB pathway. (2016) Mol.Med.Rep. 13:5379-85  WB ;  Human.  
[IF=1.56] Wang, Xuming, et al. "Characterization of sphere‑forming cells with stem‑like properties from the gastric cancer cell lines MKN45 and SGC7901." Molecular Medicine Reports 10.6 (2014): 2937-2941.  FCM ;  Human.  
[IF=1.461] Pei W et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology. 2018 Jun;70(3):1009-1021.  IF&FCM ;  ovine embryo.  
[IF=1.26] Liao et al. Bone mesenchymal stem cells co-expressing VEGF and BMP-6 genes to combat avascular necrosis of the femoral head. (2018) Exp.Ther.Med. 15:954-962  FCM ;  Rat.  
[IF=0.918] Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224.  ICF ;  lamb.  
[IF=]   ELISA, WB ;  rat.  
研究领域 肿瘤  免疫学  干细胞  细胞表面分子  糖蛋白  细胞类型标志物  t-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rabbit (predicted: Rat,Dog,Pig,Cow,Horse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 85kDa
检测分子量 80-95kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD44: 701-742/742 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008].

Function:
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Subunit:
Interacts with PKN2 (By similarity). Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.

Subcellular Location:
Membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges.

Tissue Specificity:
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Post-translational modifications:
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Similarity:
Contains 1 Link domain.

SWISS:
P16070

Gene ID:
960

Database links:

Entrez Gene: 281057 Cow

Entrez Gene: 960 Human

Entrez Gene: 12505 Mouse

Entrez Gene: 100301546 Rabbit

Entrez Gene: 25406 Rat

Omim: 107269 Human

SwissProt: Q29423 Cow

SwissProt: P16070 Human

SwissProt: P15379 Mouse

SwissProt: P26051 Rat

Unigene: 502328 Human

Unigene: 423621 Mouse

Unigene: 1120 Rat




CD44是一个重要的细胞表面粘附分子。它在许多类型的人类细胞上都表达,在很多生理和病理过程中有复杂的作用,例如细胞迁移和在肿瘤细胞的生长调控中。CD44传递着诸多的细胞通路,有研究者认为CD44是一个新的信号通路蛋白。CD44介导淋巴细胞与血管内皮细胞包括粘膜组织中高内皮小静脉的粘连,这可能是通过激活其它粘附分子而间接造成的。CD44也是肿瘤细胞浸润的重要因素。
产品图片
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: Panc-1 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-CD44 (bs-0521R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 83 kD
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (bs-0521R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD44 Polyclonal Antibody, Unconjugated(bs-0521R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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