产品中心-北京博奥森生物技术有限公司

产品编号	bs-1035R
英文名称	CD86
中文名称	CD86抗体
别名	CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Activation B7-2 antigen 3; CD86_HUMAN; Activation B7-2 antigen; Activation B72 antigen; B lymphocyte activation antigen B7 2; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7 2 antigen; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD 86; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen; CD86 molecule; CLS1; CTLA 4 counter receptor B7 2; CTLA 4 counter receptor B7.2; CTLA-4 counter-receptor B7.2 2, 3; CTLA4 counter receptor B72; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; TS/A-2.
	Specific References (63) \| bs-1035R has been referenced in 63 publications. [IF=20.722] Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359 IF ; Mouse. PubMed:10.1016/j.nantod.2021.101359 [IF=15.304] Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683 IHC ; Rat. PubMed:35870263 [IF=14.919] Singh, Alok Kumar. et al. Re-engineered BCG overexpressing cyclic di-AMP augments trained immunity and exhibits improved efficacy against bladder cancer. Nat Commun. 2022 Feb;13(1):1-16 IHC ; Mouse. PubMed:10.1038/s41467-022-28509-z [IF=14.593] Bin Wang. et al. Targeted delivery of a STING agonist to brain tumors using bioengineered protein nanoparticles for enhanced immunotherapy. Bioact Mater. 2022 Mar;: IF ; Mouse. PubMed:35386310 [IF=12.91] Kai Ye. et al. An armed oncolytic virus enhances the efficacy of tumor-infiltrating lymphocyte therapy by converting tumors to artificial antigen presenting cells in situ. MOL THER. 2022 Jun;: IHC ; Mouse. PubMed:35715953 [IF=11.092] Kun Xu. et al. Functional Titanium Substrates Synergetic Photothermal Therapy for Enhanced Antibacterial and Osteogenic Performance via Immunity Regulation. ADV HEALTHC MATER. 2023 Mar;:2300494 ICC,FCM,IHC ; Mouse. PubMed:36929688 [IF=10.435] Mei, Jiawei. et al. An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22 IF ; Mouse. PubMed:35568914 [IF=10.317] Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H 2 O 2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials . 2020 Oct;255:120155. ICF ; Mouse. PubMed:32554130 [IF=10.317] Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials. 2020 Oct;255:120155. ICF ; Mouse. PubMed:32554130 [IF=9.995] Yuqiu Xu. et al. Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers. CELL REP. 2022 Aug;40:111295 IF ; Human. PubMed:36044847 [IF=9.918] Daijun Zhou. et al. An injectable miR181a-IFI6 nanoparticles promote high-quality healing of radiation-induced skin injury. MATER TODAY ADV. 2022 Aug;15:100267 FC ; Human. PubMed:10.1016/j.mtadv.2022.100267 [IF=9.207] Li Yin. et al. G6PD activation in TNBC cells induces macrophage recruitment and M2 polarization to promote tumor progression. CELL MOL LIFE SCI. 2023 Jun;80(6):1-20 WB ; Human. PubMed:37237244 [IF=8.947] Junpeng Chen. et al. An all-in-one CO gas therapy-based hydrogel dressing with sustained insulin release, anti-oxidative stress, antibacterial, and anti-inflammatory capabilities for infected diabetic wounds. ACTA BIOMATER. 2022 Apr;: Other ; Other. PubMed:35500813 [IF=8.897] Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-10 WB ; Mouse. PubMed:10.1007/s12274-021-4050-3 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC/IF ; Rat/Mouse. PubMed:10.1016/j.apmt.2020.100673 [IF=7.525] Ndzie Noah Marie Louise. et al. Estrogen downregulates CD73/adenosine axis hyperactivity via adaptive modulation PI3K/Akt signaling to prevent myocarditis and arrhythmias during chronic catecholamines stress. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-11 IHC ; Mouse. PubMed:36823590 [IF=7.367] Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28 IF,IHC ; Mouse. PubMed:33644993 [IF=7.242] Tong Shen. et al. Sulfated chitosan rescues dysfunctional macrophages and accelerates wound healing in diabetic mice. Acta Biomater. 2020 Nov;117:192 IF ; Mouse. PubMed:33007486 [IF=6.986] Dai Shi. et al. Synthesis and Evaluation of 68Ga-NOTA-COG1410 Targeting to TREM2 of TAMs as a Specific PET Probe for Digestive Tumor Diagnosis. Anal Chem. 2022;94(9):3819–3830 ICC ; Human. PubMed:35195007 [IF=6.922] Shanping Wang. et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021; 14: 7107–7130 IF ; Mouse. PubMed:34992409 [IF=6.66] Evans, Elizabeth E., et al. "Antibody blockade of semaphorin 4D promotes immune infiltration into tumor and enhances response to other immunomodulatory therapies." Cancer Immunology Research (2015): canimm-0171. IHC-P ; Mouse. PubMed:25614511 [IF=6.543] Wang Shanping. et al. Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway. Oxid Med Cell Longev. 2022;2022:4636618 IF ; Mouse. PubMed:35126813 [IF=5.77] Sher Bahadur Poudel. et al. Excess Growth Hormone Triggers Inflammation-Associated Arthropathy, Subchondral Bone Loss, and Arthralgia. AM J PATHOL. 2023 Mar;: IHC ; Mouse. PubMed:36870529 [IF=5.763] You Shuang. et al. Recombinant humanized collagen remodels endometrial immune microenvironment of chronic endometritis through macrophage immunomodulation. REGEN BIOMATER. 2023 Apr;: IF ; Rat. PubMed:37122820 [IF=5.76] Das, Subhamoy, et al. "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." Advanced Healthcare Materials (2016). IHC-P ; Mouse. PubMed:27385307 [IF=5.714] Nancy K. El-Deeb. et al. Crosstalk between PI3K/AKT/KLF4 signaling and microglia M1/M2 polarization as a novel mechanistic approach towards flibanserin repositioning in parkinson's disease. INT IMMUNOPHARMACOL. 2022 Nov;112:109191 IHC ; Rat. PubMed:36055034 [IF=5.714] Shuling Zhang. et al. Methionine enkephalin suppresses lung cancer metastasis by regulating the polarization of tumor-associated macrophages and the distribution of myeloid-derived suppressor cells in the tumor microenvironment and inhibiting epithelial-mesenchymal transition. INT IMMUNOPHARMACOL. 2023 May;118:110064 IF ; Mouse. PubMed:36989897 [IF=5.714] Weam W. Ibrahim. et al. NLRP3 inflammasome inhibition and M1-to-M2 microglial polarization shifting via scoparone-inhibited TLR4 axis in ovariectomy/D-galactose Alzheimer's disease rat model. INT IMMUNOPHARMACOL. 2023 Jun;119:110239 IHC ; Rat. PubMed:37137264 [IF=5.7] Peruzzaro ST et al. Transplantation of mesenchymal stem cells genetically engineered to overexpress interleukin-10promotes alternative inflammatory response in rat model of traumatic brain injury. J Neuroinflammation. 2019 Jan 5;16(1):2. IHC ; Rat. PubMed:30611291 [IF=5.606] Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-3080 WB,IF ; Human. PubMed:32417913
研究领域	免疫学微生物学干细胞细胞表面分子糖蛋白淋巴细胞 t-淋巴细胞 b-淋巴细胞
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Rat,Mouse,Human (predicted: Sheep,Cow,Pig,Dog)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	31kDa
检测分子量	72-74kDa
细胞定位	细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from the middle of rat CD86: 140-175/313 <Extracellular>
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]. Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. Subunit: Homodimer. Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by activated B-lymphocytes and monocytes. Post-translational modifications: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: O35531 Gene ID: 56822 Database links: Entrez Gene: 942 Human Entrez Gene: 56822 Rat Omim: 601020 Human SwissProt: P42081 Human Unigene: 171182 Human Unigene: 229570 Rat CD86是一个很重要要的辅助刺激分子，CD86作为CD28的辅助刺激分子，提供 T 细胞活化的辅助信号,它们之间的结合和随后介导的信号转导是 T 细胞和 APC 相互协作的重要分子基础。
产品图片	Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: U937 (Human) Cell Lysate at 30 ug Lane 3: Spleen (Rat) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Lane 5: HL-60 (Human) Cell Lysate at 30 ug Primary: Anti-CD86 (bs-1035R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72-74 kD Observed band size: 72 kD Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(bs-1035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(bs-1035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记