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Rabbit Anti-c-fos antibody (bs-0469R)
~~~促销,代码KT802~~~
~~~促销,代码KT0830~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0469R
英文名称 c-fos
中文名称 c-fos抗体
别    名 Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogene homolog; AP-1; p55; FOS_HUMAN; Proto-oncogene c-Fos; G0/G1 switch regulatory protein 7.  
Specific References  (3)     |     bs-0469R has been referenced in 3 publications.
[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.  WB ;  Mouse.  
[IF=12.279] Zhang Q et al. Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer.Nano Lett . 2020 May 13;20(5):3039-3049.  WB&ICF ;  Human.  
[IF=4.4] Xiao, Jinyu, et al. "Effects of GABA microinjection into dorsal raphe nucleus on behavior and activity of lateral habenular neurons in mice." Experimental Neurology (2017).  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  转录调节因子  肿瘤细胞生物标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 41kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human c-fos:1-100/380 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Function:
Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.

Subunit:
Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activation. Interacts with MAFB. Interacts withDSIPI; this interaction inhibits the binding of active AP1 to itstarget DNA.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated in the C-terminal upon stimulation by nervegrowth factor (NGF) and epidermal growth factor (EGF).Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on bothSer-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to proteinstabilization with phosphorylation on Ser-374 being the major sitefor protein stabilization on NGF stimulation. Phosphorylation onSer-362 and Ser-374 primes further phosphorylations on Thr-325 andThr-331 through promoting docking of MAPK to the DEF domain.Phosphorylation on Thr-232, induced by HA-RAS, activates thetranscriptional activity and antagonizes sumoylation.Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes toosteoblast transformation (By similarity). [PTM] Constitutively sumoylated by SUMO1, SUMO2 and SUMO3.Desumoylated by SENP2. Sumoylation requires heterodimerization withJUN and is enhanced by mitogen stimulation. Sumoylation inhibitsthe AP-1 transcriptional activity and is, itself, inhibited byRas-activated phosphorylation on Thr-232.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

SWISS:
P01100

Gene ID:
2353

Database links:

Entrez Gene: 2353 Human

Entrez Gene: 14281 Mouse

Entrez Gene: 314322 Rat

Omim: 164810 Human

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Unigene: 246513 Mouse

Unigene: 103750 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


c-fos的作用主要用于各种类型的恶性肿瘤如食管癌、鼻咽癌、乳腺癌、结肠癌以及脑病的研究。
c-fos原癌基因及其蛋白产物不仅参与细胞的正常生长、分化过程,而且也参与细胞内信息传递过程和细胞的能量代谢过程,对细胞的增生、分化、转化都有调节作用、在生命活动中起着极为基础而重要的作用。
产品图片
MCF-7 Cell (Human) Lysate at 30 ug
Embryo(Mouse) Lysate at 40 ug
RAW246.7(Mouse)Lysate at 30 ug
NIH/3T3(Mouse)Lysate at 30 ug
Cerebrum(Mouse) Lysate at 40 ug
Cerebrum(Rat) Lysate at 40 ug
Primary: Anti- c-fos (bs-0469R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41kD
Observed band size: 55KD
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-0469R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-0469R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-0469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-0469R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-0469R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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