| 产品编号 | bs-0711R |
| 英文名称 | FAM3B Rabbit pAb |
| 中文名称 | 胰腺衍生因子抗体 |
| 别 名 | C21orf11; PANDER; Cytokine like protein 2 21; PRED44; PANcreatic DERived factor; Protein FAM3B; Protein FAM3B precursor; Family with sequence similarity 3, member B isoform a; FAM3B_MOUSE. |
| 研究领域 | 免疫学 细胞凋亡 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FAM3B: 1-100/235 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FAM3B has delayed effects on beta-cell function, inhibiting basal insulin secretion from a beta-cell line in a dose-dependent manner. [Subcellular Location] Secreted. Note=Localized in discrete vesicular and perinuclear structure. Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis. 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. Belongs to the FAM3 family. Function: Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Subcellular Location: Secreted. Note=Present in insulin secretory granules and likely cosecreted with insulin. Localized in discrete vesicular and perinuclear structure. Tissue Specificity: Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis. Post-translational modifications: 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. O-glycosylated. Similarity: Belongs to the FAM3 family. SWISS: P58499 Gene ID: 54097 Database links: Entrez Gene: 54097 Human Entrez Gene: 52793 Mouse Omim: 608617 Human SwissProt: P58499 Human SwissProt: Q9D309 Mouse Unigene: 473877 Human Unigene: 670704 Human Unigene: 327932 Mouse 胰腺衍生因子（PANcreatic DERived fa*ctor，PANDER）是新发现的一种新型的胰岛细胞因子、由胰岛α细胞和β细胞分泌的一种分泌型细胞因子，与β细胞凋亡密切相关 ，可以诱导胰岛素分泌β细胞，主要用于糖尿病的研究。 |
| 产品图片 |
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FAM3B/PANDER Polyclonal Antibody, Unconjugated(bs-0711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
