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Rabbit Anti-Fibronectin/FN1 antibody (bs-0666R)
~~~促销,代码KT2401~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0666R
英文名称 Fibronectin/FN1
中文名称 纤维连接蛋白抗体
别    名 CIG; Cold insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; ED-B; fibronectin 1; FINC; FN 1; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Transformation sensitive protein; FINC_HUMAN; Fibronectin; Cold-insoluble globulin; Ugl-Y3.  
Specific References  (25)     |     bs-0666R has been referenced in 25 publications.
[IF=32.086] Ali Doryab. et al. Real-Time Measurement of Cell Mechanics as a Clinically Relevant Readout of an In Vitro Lung Fibrosis Model Established on a Bioinspired Basement Membrane. ADV MATER. 2022 Sep;:2205083  IF ;  Human.  
[IF=10.612] Yosuke Nagai. et al. Rho-associated, coiled-coil–containing protein kinase 1 regulates development of diabetic kidney disease via modulation of fatty acid metabolism. KIDNEY INT. 2022 May;:  IF ;  Mouse.  
[IF=10.19] Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333  IHC ;  Human.  
[IF=6.291] Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634  IHC ;  Mouse.  
[IF=5.116] Siwen Zhang. et al. Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res Ther. 2021 Dec;12(1):1-16  IF,IHC ;  Rat.  
[IF=4.522] Fasano A et al. HTRA1 expression profile and activity on TGF‐β signaling in HTRA1 mutation carriers. J Cell Physiol. 2020 Feb 4.  WB ;  Human.  
[IF=4.427] Wu M et al. Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/transforming growth factor β type II receptor axis.(2018) Chemosphere 219  ICF ;  Human.  
[IF=4.2] Miyauchi, Yuya, et al. "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." Scientific Reports 7 (2017).  IHC-P ;  Rat.  
[IF=4.12] Wu et al. MicroRNA-27a Induces Mesangial Cell Injury by Targeting of PPARγ, and its In Vivo Knockdown Prevents Progression of Diabetic Nephropathy. (2016) Sci.Rep. 6:26072  WB ;  Rat.  
[IF=3.87] Ma, Yan, et al. "Intraocular expression of serum amyloid a and interleukin-6 in proliferative diabetic retinopathy." American journal of ophthalmology 152.4 (2011): 678-685.  IHC-F ;  Mouse.  
[IF=3.848] Song Danyang. et al. Silencing of ER-resident oxidoreductase PDIA3 inhibits malignant biological behaviors of multidrug-resistant gastric cancer. Acta Bioch Bioph Sin. 2021 Aug;:  IHC ;  Human.  
[IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062.  IHC-P ;  Rat.  
[IF=3.61] Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722.  IHC-P ;  Mouse.  
[IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).  WB ;  Mouse.  
[IF=3.269] Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-11200  WB ;  Rat.  
[IF=2.61] Jun, Hao, et al. "In vivo and in vitro effects of SREBP-1 on diabetic renal tubular lipid accumulation and RNAi-mediated gene silencing study." Histochemistry and cell biology 131.3 (2009): 327-345.  WB ;  Rat.  
[IF=2.51] Wu, Lina, et al. "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." Molecular biology reports 39.9 (2012): 9085-9093.  IHC-P ;  Rat.  
[IF=2.33] Elmas, Onur, Oytun Erbas, and Gurkan Yigitturk. "The efficacy of Aesculus hippocastanum seeds on diabetic nephropathy in a streptozotocin-induced diabetic rat model." Biomedicine & Pharmacotherapy 83 (2016): 392-396.  IHC-F ;  Rat.  
[IF=2.2] Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.  WB ;  Bovine.  
[IF=2.17] Bai, X. P., et al. "Fabrication of engineered heart tissue grafts from alginate/collagen barium composite microbeads." Biomedical Materials 6.4 (2011): 045002.  IHC-P ;  Rat.  
[IF=2.067] H-J Wang. et al. MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy:. Hum Exp Toxicol. 0;():  WB ;  Human.  
[IF=1.785] Xiaoyan Yu. et al. Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase. Exp Ther Med. 2021 Apr;21(4):1-1  ELISA ;  Rat.  
[IF=1.238] Liu and Xiao Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia. (2017) BMC.Ophthalmo. 17:135  WB ;  Human.  
[IF=0.857] Wang YP et al. Effects of Qingshen granules on Janus Kinase/ signal transducer and activator of transcription signaling pathway in rats with unilateral ureteral obstruction.J Tradit Chin Med. 2018 April 15; 38(2): 182-189.  IHC-P ;  Rat.  
[IF=0.78] Karadeniz, Tugba, et al. "Experimental comparison of protective characteristics of enalapril and trimetazidine in diabetic nephropathy." Renal failure 36.8 (2014): 1283-1290.  IHC-P ;  Mouse.  
研究领域 肿瘤  细胞生物  免疫学  信号转导  转录调节因子  细胞粘附分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Cow,  (predicted: Dog, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:200-800 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 259kDa
细胞定位 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Fibronectin/Ugl-Y3: 1201-1300/2386 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].

Function:
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Subunit:
Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7. Interacts with COMP. Interacts with S.aureus fnbA. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth. Interacts with FST3.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Post-translational modifications:
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylation sites are present in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

DISEASE:
Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life.

Similarity:
Contains 12 fibronectin type-I domains. Contains 2 fibronectin type-II domains. Contains 16 fibronectin type-III domains.

SWISS:
P11276

Gene ID:
2335

Database links:

Entrez Gene: 280794 Cow

Entrez Gene: 2335 Human

Entrez Gene: 14268 Mouse

Entrez Gene: 25661 Rat

Omim: 135600 Human

SwissProt: P07589 Cow

SwissProt: P02751 Human

SwissProt: P11276 Mouse



细胞粘附蛋白(Call Adhesion Protein)
FaN(Fibronectin)是一种细胞粘附蛋白,参与细胞粘附,分化,移动,伤口愈合,凝血和机体防卫。主要分布于结缔组织及大部分基底膜上,作为判断良恶性上皮源性肿瘤(如乳腺癌等)的一个主要参考指标,亦可作为恶性肿瘤转移和病人预后的判断指标
产品图片
Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated(bs-0666R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fibronectin) Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated(bs-0666R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-Fibronectin Polyclonal Antibody, conjugated (bs-0666R-AF647) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
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