扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-Galectin 3  antibody (bs-0721R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0721R
英文名称 Galectin 3
中文名称 半乳糖凝集素3抗体
别    名 Galectin-3; 35 kDa lectin; Carbohydrate binding protein 35; CBP 35; CBP35; GAL 3; GAL3; GAL-3; Galactose specific lectin 3; Galactose-specific lectin 3; Galactoside binding protein; Galactoside-binding protein; GALBP; Galectin3 internal gene,included; GALIG; GBP; IgE binding protein; IgE-binding protein; L 31; L 34; L-34 galactoside-binding lectin; L31; Laminin binding protein; Laminin-binding protein; Lectin galactose binding soluble 3; Lectin galactoside binding soluble 3; Lectin L 29; Lectin L-29; Lectin, galactose binding, soluble 3; LGALS 3; LGALS2; LGALS3; LGALS3 protein; MAC 2 antigen; Mac-2; MAC2; Macrophage galactose-specific lectin; MGC105387; LEG3_HUMAN.  
Specific References  (5)     |     bs-0721R has been referenced in 5 publications.
[IF=5.36] White, Michael JV, David Roife, and Richard H. Gomer. "Galectin-3 Binding Protein Secreted by Breast Cancer Cells Inhibits Monocyte-Derived Fibrocyte Differentiation." The Journal of Immunology (2015): 1500365.  other ;  Human.  
[IF=3.218] Liu H et al. Dual-signal sandwich-type electrochemical immunoassay of galectin-3 using methylene blue and gold nanoparticles biolabels. Journal of Electroanalytical Chemistry, 113952 (2020).  Other ;  Human.  
[IF=2.824] Xu Mei. et al. Trichinella spiralis co-infection exacerbates Plasmodium berghei malaria-induced hepatopathy. Parasite Vector. 2020 Dec;13(1):1-16  IHC ;  Mouse.  
[IF=2.663] Zeng Y et al. Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.(2018) Lipids in Health and Disease, Nov 6;17(1):251.  IHC ;  Mouse.  
[IF=1.08] Lee, Ju Yeon, et al. "Molecular cloning and expression analysis of pig CD7." Veterinary Research Communications (2014): 1-7.  Pig.  
研究领域 肿瘤  免疫学  细胞凋亡  细胞粘附分子  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse
产品应用 IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 29kDa
细胞定位 细胞核 细胞浆 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Galectin 3: 151-250/250 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010].

Function:
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Subunit:
Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.

Subcellular Location:
Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface.

Tissue Specificity:
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.

Similarity:
Contains 1 galectin domain.

SWISS:
P17931

Gene ID:
3958

Database links:

Entrez Gene: 3958 Human

Entrez Gene: 16854 Mouse

Entrez Gene: 83781 Rat

Omim: 153619 Human

SwissProt: P17931 Human

SwissProt: P16110 Mouse

SwissProt: P08699 Rat

Unigene: 531081 Human

Unigene: 248615 Mouse

Unigene: 764 Rat



Galectin-3是Galectin(半乳凝素)家族的一员,能识别糖蛋白和糖脂的特异性低聚糖结构,参与多种生理和病理过程,包括细胞生长和凋亡、细胞黏附、新生血管形成和肿瘤浸润与转移等。Galectin-3在肿瘤的发生、发展及转移中起重要作用。
Galectin-3在某些肿瘤组织中高表达,其在不同类型肿瘤,如结直肠癌、胃癌、胰腺癌、甲状腺癌及肺癌等患者中的高表达水平,与低生存、肿瘤复发、预后差及对化疗药物敏感性降低相关对于某些肿瘤,Galectin-3有可能成为一个有价值的诊断标志和治疗靶点。
产品图片
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galectin 3 Polyclonal Antibody, Unconjugated(bs-0721R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Cervical Cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-0721R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galectin 3 Polyclonal Antibody, Unconjugated(bs-0721R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-Galectin 3 antibody (bs-0721R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版权所有 2004-2026 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    认证编号: 00122Q31509R1M/1100
京ICP备05066980号-1         京公网安备110107000727号