扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Mouse Anti-GAPDH (Loading Control) antibody (bsm-0978M)
~~~促销,代码SQ220725~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  500ul
50ul/680.00元
100ul/980.00元
500ul/4000.00元
大包装/询价

产品编号 bsm-0978M
英文名称 GAPDH (Loading Control)
中文名称 3-磷酸甘油醛脱氢酶(内参)单克隆抗体
别    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
Specific References  (74)     |     bsm-0978M has been referenced in 74 publications.
[IF=2.248] Wang Yulong. et al. LncRNA GAS5 promotes abdominal aortic aneurysm formation through regulating the miR-185-5p/ADCY7 axis. Anti-Cancer Drug. 2022 Mar;33(3):225-234  WB ;  Human.  
[IF=3.139] Ting Chen. et al. Overexpression of p53 accelerates puberty in high-fat diet–fed mice through Lin28/let-7 system:. Exp Biol Med. 2020;246(1):66-71  WB ;  Mouse.  
[IF=5.191] Lingyan Ren. et al. Inflammatory osteoclasts-derived exosomes promote bone formation by selectively transferring lncRNA LIOCE into osteoblasts to interact with and stabilize Osterix. Faseb J. 2022 Feb;36(2):e22115  WB ;  Mouse.  
[IF=4.105] Ai Jin. et al. TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts. Bba-Mol Cell Res. 2021 Jun;:119083  WB ;  Human.  
[IF=3.943] Xiaoyue Guan. et al. Crosstalk between Wnt/β-catenin signaling and NF-κB signaling contributes to apical periodontitis. Int Immunopharmacol. 2021 Sep;98:107843  WB ;  Human.  
[IF=3.033] Hui Wang. et al. The decreased expression of integrin αv is involved in T-2 toxin-induced extracellular matrix degradation in chondrocytes. Toxicon. 2021 Aug;199:109  WB ;  Human.  
[IF=3.052] Yilei Jing. et al. Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity. J Chem Neuroanat. 2021 Nov;117:102004  WB ;  Rat.  
[IF=2.571] Jin-Yong Mu. et al. LncRNA RBM5-AS1 promotes cell proliferation and invasion by epigenetically silencing miR-132/212 in hepatocellular carcinoma cells. 2021 May 21  WB ;  Human.  
[IF=3.943]   WB ;  mouse.  
[IF=3.647]   WB ;  human.  
[IF=3.448]   
[IF=6.551] Gaolong Zhong. et al. Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks. Sci Total Environ. 2021 Sep;788:147780  WB ;  Duck.  
[IF=3.943] Yuntai Shen. et al. Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury. Int Immunopharmacol. 2020 Dec;89:107005  WB ;  Mouse.  
[IF=5.115] Xia Qinet al. Zinc Oxide Nanoparticles Induce Ferroptotic Neuronal Cell Death in vitro and in vivo. Int J Nanomedicine . 2020 Jul 27;15:5299-5315.  IF ;  mouse.  
[IF=4.556] Bobae Kimet al. B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues. Int J Mol Sci . 2020 Jul 20;21(14):5121.  WB ;  mouse.  
[IF=3.03] He S et al. Avian leukosis virus subgroup J induces B cell anergy mediated by Lyn inhibited BCR signal transduction. Vet Microbiol. 2020 Aug;247:108781.  WB ;  Chicken.  
[IF=6.162] Zhang W et al. Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine. Cancers (Basel). 2020 Jan 29;12(2). pii: E311.  WB ;  Human.  
[IF=3.414] Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.  WB ;  Human.  
[IF=4.259] Qu S et al. Cinnamaldehyde, a Promising Natural Preservative Against Aspergillus flavus. Front Microbiol. 2019 Dec 18;10:2895.  WB ;  A. flavus.  
[IF=1.832] Gao J et al. Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep.Animals (Basel). 2019 May 31;9(6). pii: E309.  WB ;  Sheep.  
[IF=2.35] Yasukochi A et al. Regulation of collagen type XVII expression by miR203a-3p in oral squamous cell carcinomacells. J Biochem. 2019 Mar 27. pii: mvz024.  WB ;  Human.  
[IF=0] Usuki S et al. Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism. Biochem Biophys Rep. 2019 Jan 3;17:132-138.  WB ;  Human.  
[IF=1.166] Wang YN et al. Expression of visual system homeobox 1 in human keratoconus. Int J Ophthalmol. 2019 Feb 18;12(2):201-206.  WB ;  Human.  
[IF=0] Fahie, Monifa, "Pore forming protein assembly and the use in nanopore sensing: a study on E. coli proteins ClyA and OmpG" (2017). Doctoral Dissertations. 1076.  WB ;  
[IF=4.44] Katoh, Hiroshi, et al. "Heat shock protein 90 ensures efficient mumps virus replication by assisting with viral polymerase complex formation." Journal of Virology (2017): JVI-02220.  WB ;  Monkey.  
[IF=3.03] Liu, Si-jun, et al. "Neuroprotective effect of β-asarone against Alzheimer’s disease: regulation of synaptic plasticity by increased expression of SYP and GluR1." Drug design, development and therapy 10 (2016): 1461.  WB ;  Rat.  
[IF=2.09] Di, Yu, et al. "CCN1/Cyr61-PI3K/AKT signaling promotes retinal neovascularization in oxygen-induced retinopathy." International journal of molecular medicine 36.6 (2015): 1507-1518.  WB ;  Human, Mouse.  
[IF=1.27] Liu, Yong, et al. "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." Experimental and Therapeutic Medicine.  WB ;  Rabbit.  
[IF=0.65] Xu, Xiaochun, et al. "PPARγ, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development." Electronic Journal of Biotechnology (2015).  WB ;  Sheep.  
[IF=2.38] Zhang, Xiao‐Qun, et al. "Regulation of pulmonary surfactant synthesis in fetal rat type II alveolar epithelial cells by microRNA‐26a." Pediatric Pulmonology (2014).  WB ;  Rat.  
[IF=2.8] Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013).  WB ;  Rat.  
[IF=5.88] Shuai Chen. et al. Tenogenic adipose-derived stem cell sheets with nanoyarn scaffolds for tendon regeneration. Mat Sci Eng C-Mater. 2021 Feb;119:111506  WB ;  Rabbit.  
[IF=4.534] Ji-Lun Cheng. et al. LncRNA KCNQ1OT1 regulates the invasion and migration of hepatocellular carcinoma by acting on S1PR1 through miR-149. Cancer Gene Ther. 2020 Aug;:1-14  WB ;  Human.  
[IF=4.872] Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:112350  WB ;  Duck.  
[IF=13.281] Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 20  WB,IF ;  Human; Mouse.  
[IF=3.06] Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 20  WB ;  Rat.  
[IF=6.244] Pei Dai. et al. Gimap5 Inhibits Lung Cancer Growth by Interacting With M6PR. Front Oncol. 2021; 11: 699847  WB ;  Human.  
[IF=5.187] Mingzhu Jiang. et al. Blocking AURKA with MK-5108 attenuates renal fibrosis in chronic kidney disease. Bba-Mol Basis Dis. 2021 Nov;1867:166227  WB ;  Rat.  
[IF=4.486] Zhuohong Li. et al. Anlotinib exerts anti-cancer efficiency on lung cancer stem cells in vitro and in vivo through reducing NF-κB activity. 2021 May 06  WB ;  Mouse.  
[IF=3.056] Shaofeng Wu. et al. Evaluation of toxic effects induced by arsenic trioxide or/and antimony on autophagy and apoptosis in testis of adult mice. 2021 May 20  WB ;  Mouse.  
[IF=4.225] Hao Ding. et al. Effects of Real-Ambient PM2.5 Exposure on Lung Damage Modulated by Nrf2−/−. Front Pharmacol. 2021; 12: 662664  WB ;  Mouse.  
[IF=2.363] Wang Li. et al. Trilobatin Induces Apoptosis and Attenuates Stemness Phenotype of Acquired Gefitinib Resistant Lung Cancer Cells via Suppression of NF-κB Pathway. 2021 Apr 16  WB ;  Human.  
[IF=3.876] Daisuke Fujioka. et al. Group V Secretory Phospholipase A2 Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line. 2021 Mar 27  WB ;  Mouse.  
[IF=5.606] Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-3080  WB ;  Human.  
[IF=5.201] Guo Qiang. et al. PAQR3 Inhibits Non-small Cell Lung Cancer Growth by Regulating the NF-κB/p53/Bax Axis. Front Cell Dev Biol. 2020 Oct;8:1060  WB ;  Human.  
[IF=3.375] Cuie Gao. et al. Pathogenic role of circulating CD4+CXCR5+ cell subpopulations in patients with chronic spontaneous urticarial.. Am J Transl Res. 2020; 12(8): 4434–4444  WB ;  Human.  
[IF=3.267] Seona Cho. et al. Viburnum stellato-tomentosum Extract Suppresses Obesity and Hyperglycemia through Regulation of Lipid Metabolism in High-Fat Diet-Fed Mice. Molecules. 2021 Jan;26(4):1052  WB ;  Mouse.  
[IF=3.212] Gaolong Zhong. et al. The protective role of autophagy against arsenic trioxide-induced cytotoxicity and ROS-dependent pyroptosis in NCTC-1469 cells. J Inorg Biochem. 2021 Apr;217:111396  WB ;  Mouse.  
[IF=6.551] Yukun Fang. et al. Activation of the ROS/HO-1/NQO1 signaling pathway contributes to the copper-induced oxidative stress and autophagy in duck renal tubular epithelial cells. Sci Total Environ. 2021 Feb;757:143753  WB ;  Duck.  
[IF=1.931] Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034  WB ;  Human.  
[IF=6.551] Gaolong Zhong. et al. Arsenic or/and antimony induced mitophagy and apoptosis associated with metabolic abnormalities and oxidative stress in the liver of mice. Sci Total Environ. 2021 Jul;777:146082  WB ;  Mouse.  
[IF=3.998] Xiao-Yi Zhang. et al. Dietary capsaicin normalizes CGRP peptidergic DRG neurons in experimental diabetic peripheral neuropathy. Sci Rep-Uk. 2021 Jan;11(1):1-19  WB ;  Rat.  
[IF=3.297] Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127  WB ;  Rat.  
[IF=2.842] Chen T et al. p53 Mediates GnRH Secretion via Lin28/let-7 System in GT1-7 CellsDiabetes Metab Syndr Obes.2020 Dec 1;13:4681-4688.  WB ;  Mouse.  
[IF=3.647] Weiyi Zhanget al. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci . 2020 Oct 1;258:118151.  other ;  
[IF=3.337] Xuesong Wanget al. LncRNA NEAT1 Regulates 5-Fu Sensitivity, Apoptosis and Invasion in Colorectal Cancer Through the MiR-150-5p/CPSF4 Axis. Onco Targets Ther . 2020 Jul 1;13:6373-6383.  WB ;  Human.  
[IF=2.13] Wei, Ting, et al. "The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma." Experimental and molecular pathology (2012).  WB ;  Human.  
[IF=2.984] Ming Lyuet al. Overexpression of the Tuberous sclerosis complex 2 (TSC2) gene inhibits Goat myoblasts proliferation and differentiation in understanding the underlying mechanism of muscle development. Gene . 2020 Oct 5;757:144943.  WB ;  Goat.  
[IF=3.03] Shuhai Heet al. Avian leukosis virus subgroup J induces B cell anergy mediated by Lyn inhibited BCR signal transduction. Vet Microbiol . 2020 Aug;247:108781.  WB, IHC ;  chicken.  
[IF=5.201] Cong Wanget al. Disruption of FGF Signaling Ameliorates Inflammatory Response in Hepatic Stellate Cells. Front Cell Dev Biol . 2020 Jul 22;8:601.  WB ;  Human.  
[IF=5.026] Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKIIα-associated activation of autophagy. Cell Biosci . 2020 Jul 30;10:90.  WB ;  Human.  
[IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268.  WB ;  Human.  
[IF=3.266] Wang F et al. Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. Am J Transl Res. 2019 Nov 15;11(11):7166-7185.  WB ;  Human.  
[IF=4.784] Jin Y et al. Cancer-associated mutation abolishes the impact of TRIM21 on the invasion of breast cancer cells. Int J Biol Macromol. 2019 Oct 14. pii: S0141-8130(19)34947-5.  WB ;  Human.  
[IF=2.807] Ma WJ et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2019 Aug 13. pii: S2210-7401(19)30173-1.  WB ;  Rabbit.  
[IF=4.427] Zhang H et al. Aluminum trichloride-induced hippocampal inflammatory lesions are associated with IL-1β-activated IL-1 signaling pathway in developing rats. Chemosphere. 2018 Jul;203:170-178.  WB ;  Rat.  
[IF=1.894] Liu et al. Inhibition of Expression of the S100A8 Gene Encoding the S100 Calcium-Binding Protein A8 Promotes Apoptosis by Suppressing the Phosphorylation of Protein Kinase B (Akt) in Endometrial Carcinoma and HEC-1A Cells. (2018) Med.Sci.Monit. 24:1836-1846  WB ;  Human.  
[IF=1.58] Kim et al. Relaxin Receptor RXFP1 and RXFP2 Expression in Ligament, Tendon, and Shoulder Joint Capsule of Rats. (2016) J.Korean.Med.Sci. 31:983-8  WB ;  Rat.  
[IF=1.559] Zhou et al. miR612 is associated with esophageal squamous cell carcinoma development and metastasis, mediated through TP53. (2017) Mol.Med.Re. 16:1855-1863  WB ;  Human.  
[IF=1.3] Usuki, Seigo, et al. "Characterization of Konjac Ceramide (kCer) Binding to Sema3A Receptor Nrp1." Journal of oleo science (2017): ess17142.  WB ;  Rat.  
[IF=3.33] Zhao, Hai-hua, et al. "Involvement of GSK3 and PP2A in ginsenoside Rb1's attenuation of aluminum-induced tau hyperphosphorylation." Behavioural Brain Research (2012).  WB ;  Mouse.  
[IF=2.86] Pei, Haitao, et al. "The Effect of Minimally Invasive Hematoma Aspiration on the JNK Signal Transduction Pathway after Experimental Intracerebral Hemorrhage in Rats." International journal of molecular sciences 17.5 (2016): 710.  WB ;  Rat.  
[IF=3.08] Cui, Yanjun, and Xianhong Gu. "Proteomic changes of the porcine small intestine in response to chronic heat stress." Journal of Molecular Endocrinology (2015): JME-15.  WB ;  
[IF=5.62] Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014).  WB ;  Rat.  
产品类型 内参抗体 
研究领域 肿瘤  免疫学  神经生物学  信号转导  通道蛋白  新陈代谢  Alzheimer's  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 3E12
交叉反应 Human, Mouse, Rat,  (predicted: Rabbit, )
产品应用 WB=1:1000-5000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 38kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Full length GAPDH protein purified from rabbit muscle 
亚    型 IgG
纯化方法 affinity purified by Protein G
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

SWISS:
P46406

Gene ID:
100009074

Database links:

Entrez Gene: 374193 Chicken

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: P00356 Chicken

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P04797 Rat

SwissProt: Q5XJ10 Zebrafish



GAPDH蛋白几乎在所有组织中都高水平表达,广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。
GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中,可能存在总蛋白浓度测定不准确;或者蛋白质样品在电泳前上样时产生的样品间的操作误差;这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正,所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除,得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。
甘油醛-3-磷酸脱氢酶(Glyceraldehyde 3 phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)过程中的关键酶。除了在胞质中作为糖酵解的酶以外,有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程,包括膜融合(membrane fusion)、微管成束(microtubule bundling)、磷酸转移酶(phosphotransferase)激活、核内RNA出核、DNA复制与DNA修复。一些生理因素,诸如低氧(hypoxia)和尿糖(diabetes),可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中,以高水平持续表达。
GAPDH(甘油醛-3-磷酸脱氢酶)是参与糖酵解的一种关键酶,由4个30-40kDa的亚基组成.
产品图片
Sample:
Placenta (Mouse) Lysate at 40 ug
293T Cell Lysate at 40 ug
Primary: Anti-GAPDH(bsm-0978M)at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Sample: Bladder(Rat)Lysate at 40 ug
Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Sample:
A549(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- GAPDH(3E12) (bsm-0978M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Sample:Liver(Mouse)Lysate at 40 ug
Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Lane 4: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (bsm-0978M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH(3E12)-Loading Control (bsm-0978M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH(3E12)-Loading Control) Polyclonal Antibody, Unconjugated (bsm-0978M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH(3E12)-Loading Control) Polyclonal Antibody, Unconjugated (bsm-0978M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0296G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
版权所有 2019-2022 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号