| 产品编号 | bs-0201R |
| 英文名称 | GDNFRA Rabbit pAb |
| 中文名称 | GDNF家族受体α-1抗体 |
| 别 名 | GDNF Receptor alpha 1; GFRa-1; GFR alpha; GDNFR alpha; TmR1; GFR alpha-1; RETL1; RET ligand 1; TGF-beta related neurotrophic factor receptor-1; AU042498; DKFZp313E1012; DKFZp686J0156; FLJ10561; FLJ31546; GDNFR; Gdnfr alpha; GFR-ALPHA-1; GFRA1; GRFA1; LOC143381; MGC23045; Ret; RET1L; RETL1; TRNR1; GFRA1_HUMAN; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; TGF-beta-related neurotrophic factor receptor 1. TGF-β related neurotrophic factor receptor-1; |
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Specific References (2) | bs-0201R has been referenced in 2 publications.
[IF=5.432] Lin Liu. et al. Aberrantly expressed GFRα-1/RET in patients with lacrimal adenoid cystic carcinoma is associated with high recurrence risk: a retrospective study of 51 LACC cases. Cancer Biol Med. 2021 Feb 15; 18(1): 199–205 IHC ; Human.
[IF=5.23] Ma, Fanglin, et al. "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." Scientific Reports 6 (2016): 38805. IF(ICC) ; Goat.
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| 研究领域 | 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GFR Alpha-1: 351-429/465 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq] Function: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Subunit: 2 molecules of GDNFR-alpha are thought to form a complex with the disulfide-linked GDNF dimer and with 2 molecules of RET. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Similarity: Belongs to the GDNFR family. SWISS: P56159 Gene ID: 2674 Database links: Entrez Gene: 2674 Human Entrez Gene: 14585 Mouse Omim: 601496 Human SwissProt: P56159 Human SwissProt: P97785 Mouse Unigene: 591913 Human Unigene: 88489 Rat GFRα-1是由68个氨基酸组成的蛋白，广泛表达于中枢神经系统和外周器官。活性亚单位由两个GFRα-1分子中间由二硫键连接而成的复合物。属于GDNFR 家族成员。 |
| 产品图片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MOLT4 cell lysates
Lane 4: Human A549 cell lysates
Primary: Anti-GDNFRA (bs-0201R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-GDNFRA (bs-0201R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Human colon cancer ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GDNFRA Polyclonal Antibody, Unconjugated(bs-0201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
