| 产品编号 | bs-0455R |
| 英文名称 | HSL/LIPE |
| 中文名称 | 荷尔蒙敏感脂肪酶抗体 |
| 别 名 | Hormone sensitive lipase; hormone-sensitive lipase; Hormone sensitive lipase testicular isoform; HSL; LHS; Lipase hormone sensitive; LIPE; Hormone-sensitive lipase; LIPS_HUMAN. |
 |
Specific References (1) | bs-0455R has been referenced in 1 publications.
[IF=2.742] Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7 WB ; Mouse.
|
| 研究领域 | 心血管 免疫学 生长因子和激素 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Fruit Fly) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 116kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat HSL: 261-360/1068 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HSL ( hormone- sensitive lipase ) is an enzyme involved in hydrolysis of stored triglycerides and fatty acids; important for regulation of energy homeostasis. It has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Function: In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Subunit: Interacts with PTRF in the adipocyte cytoplasm. Subcellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Similarity: Belongs to the 'GDXG' lipolytic enzyme family. SWISS: P15304 Gene ID: 25330 Database links: Entrez Gene: 3991 Human Entrez Gene: 16890 Mouse Omim: 151750 Human SwissProt: Q05469 Human SwissProt: Q6NSL7 Human SwissProt: P54310 Mouse Unigene: 656980 Human Unigene: 158548 Mouse Unigene: 333679 Mouse Unigene: 10566 Rat HSL又称：敏感性甘油三酯脂肪酶，在脂肪动员中，脂肪细胞内激素敏感性甘油三酯脂肪酶（HSL）起决定性作用，它是脂肪分解的限速酶，称为激素敏感性脂肪酶。 肾上腺素、去甲肾上腺素、胰高血糖素等激活HSL促进脂肪动员；胰岛素，前列腺素E及烟酸等抑制HSL，抑制脂肪的动员。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSL LIPE) Polyclonal Antibody, Unconjugated (bs-0455R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
