| 产品编号 | bs-1024R |
| 英文名称 | GDNF |
| 中文名称 | 胶质细胞源性神经营养因子抗体 |
| 别 名 | glial cell line derived neurotrophic factor; Astrocyte derived trophic factor 1; Astrocyte derived trophic factor; ATF 1; ATF 2; ATF1; ATF2; Glial cell derived neurotrophic factor; Glial derived neurotrophic factor; HFB1 GDNF; GDNF_HUMAN; Atf; gdnf; Glial Cell Line Derived Neurotrophic Factor; Glial cell line-derived neurotrophic factor; Glial derived neurotrophic factor; hGDNF; HSCR3. |
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Specific References (5) | bs-1024R has been referenced in 5 publications.
[IF=5.984] Zhang N et al. GLAST‐CreERT2 mediated deletion of GDNF increases brain damage and exacerbates long‐term stroke outcomes after focal ischemic stroke in Mousemodel. Glia. 2020 Nov;68(11):2395-2414. WB ; Mouse.
[IF=5.546] Min Ai. et al. 1,25(OH)2D3 attenuates sleep disturbance in mouse models of Lewis lung cancer, in silico and in vivo. 2021 Jun 01 WB ; Mouse.
[IF=2.472] Fei J et al. Electroacupuncture promotes peripheral nerve regeneration after facial nerve crush injury and upregulates the expression of glial cell-derived neurotrophic factor. Neural Regen Res. 2019 Apr;14(4):673-682. WB ; Rabbit.
[IF=2.299] Jiang Y et al. Transcriptome profile of bovine iPSCs derived from Sertoli Cells. Theriogenology. 2019 Nov 19. pii: S0093-691X(19)30522-9. FCM ; Holstein bull.
[IF=0.14] Zhang, R., et al. "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." Neural Regeneration Research 9.3 (2014): 301. IHC-P ; Rat.
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| 研究领域 | 神经生物学 细胞凋亡 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Pig,Dog,Chicken) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDNF: 121-211/211 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neurobiology. Neurotrophins. Neuroscience. This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3) [MIM:613711]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Similarity: Belongs to the TGF-beta family. GDNF subfamily. SWISS: P39905 Gene ID: 2668 Database links: Entrez Gene: 2668 Human Entrez Gene: 14573 Mouse Omim: 600837 Human SwissProt: P39905 Human SwissProt: P48540 Mouse Unigene: 248114 Human Unigene: 4679 Mouse Unigene: 53970 Rat 神经生物学相关蛋白 |
| 产品图片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Spinal cord (Mouse) Lysate at 40 ug Lane 3: Cerebrum (Rat) Lysate at 40 ug Lane 4: Spinal cord (Rat) Lysate at 40 ug Lane 5: U87MG (Human) Cell Lysate at 30 ug Lane 6: U251 (Human) Cell Lysate at 30 ug Primary: Anti-GDNF (bs-1024R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25/20 kD Observed band size: 28 kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GDNF Polyclonal Antibody, Unconjugated(bs-1024R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
