扫码关注公众号           扫码咨询技术支持
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-Growth hormone receptor antibody (bs-0654R)
~~~促销,代码KT802~~~
~~~促销,代码KT0830~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0654R
英文名称 Growth hormone receptor
中文名称 生长激素受体抗体
别    名 GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.  
Specific References  (10)     |     bs-0654R has been referenced in 10 publications.
[IF=1.27] Song, Cheng-Jun, et al. "Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes." International Journal of Clinical and Experimental Medicine 8.7 (2015): 10411-10419.  WB ;  Rat.  
[IF=.86] Zhou et al. Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets. (2018) Asian-Australas.J.Anim.Sci. 31:32-39  IHC-P ;  Pig.  
[IF=4.24] Hetz, Jennifer A., et al. "Growth axis maturation is linked to nutrition, growth and developmental rate." Molecular and Cellular Endocrinology (2015).  WB ;  
[IF=4.12] Cui et al. Generation of a miniature pig disease model for human Laron syndrome. (2015) Sci.Rep. 5:15603  WB ;  Porcine.  
[IF=3.86] Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).  WB ;  Human.  
[IF=2.51] Yang, Hai Li, et al. "Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte." Molecular biology reports 39.9 (2012): 9113-9122.  WB ;  Pig.  
[IF=2.445] Xie W et al. Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-likegrowth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus). Gen Comp Endocrinol. 2019 May 20;281:58-66.   IHC-P&WB ;  muskrat.  
[IF=2.23] Chen et al. Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis. (2013) Neural.Regen.Res. 8:1756-64  WB ;  Rat.  
[IF=2.02] Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310.  IHC-P ;  Rat.  
[IF=1.84] Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016).  WB ;  Sheep.  
研究领域 细胞生物  发育生物学  信号转导  生长因子和激素  细胞分化  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 68kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GHR:101-200/451 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011].

Function:
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.

Subunit:
On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain. Binding to SOCS3 inhibits JAK2 activation, binding to CIS and SOCS2 inhibits STAT5 activation. Interacts with ADAM17.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway.
Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=Remains fixed to the cell membrane and is not internalized.
Growth hormone-binding protein: Secreted. Note=Complexed to a substantial fraction of circulating GH.

Tissue Specificity:
Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

Post-translational modifications:
The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.

DISEASE:
Laron syndrome (LARS) [MIM:262500]: A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Note=The disease is caused by mutations affecting the gene represented in this entry.
Idiopathic short stature autosomal (ISSA) [MIM:604271]: Short stature is defined by a subnormal rate of growth. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 1 fibronectin type-III domain.

SWISS:
P10912

Gene ID:
2690

Database links:

Entrez Gene: 2690 Human

Entrez Gene: 14600 Mouse

Entrez Gene: 25235 Rat

Omim: 600946 Human

SwissProt: P10912 Human

SwissProt: P16882 Mouse

SwissProt: P16310 Rat

Unigene: 125180 Human

Unigene: 684631 Human

Unigene: 3986 Mouse

Unigene: 2178 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

生长激素受体(growth hormone receptor,GHR)是细胞因子/造血因子受体超级家族成员之一。
生长激素受体通过二聚体的形式和生长激素(growth hormone, GH)相结合,然后诱发Janus 激酶2 (Janus kinase 2,JAK2:是很多细胞因子、生长因子及干扰素的重要信号传感器)等细胞因子酪氨酸磷酸化并通过4条不同的途径将信号传入细胞内从而产生一系列的生理效应。
产品图片
Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Testis (Rat) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Growth hormone receptor (bs-0654R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130/110 kD
Observed band size: 130 kD
This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:300 followed by conjugation to a secondary antibody and staining.
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GHR Polyclonal Antibody, Unconjugated(bs-0654R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) .
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).
Positive control: A549 cells
Concebtration: 1μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
版权所有 2019-2022 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号