| 产品编号 | bs-0098R |
| 英文名称 | GIP Rabbit pAb |
| 中文名称 | 胃泌素抑制肽抗体 |
| 别 名 | Gastric Inhibitory polypeptide; Glucose dependent insulinotropic polypeptide; Gastric Inhibitory Peptide; GIP_HUMAN; Glucose-dependent insulinotropic polypeptide; Incretin hormone. |
| 研究领域 | 信号转导 生长因子和激素 转录调节因子 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Pig,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 5/17 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GIP: 52-93/153 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GIP (Gastric Inhibitory polypeptide ) belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. Mature GIP is a highly conserved 42 amino acid polypeptide belonging to the glucagon family. GIP is highly expressed as a pre-pro-protein in the duodenum and the jejunum. The mature secreted polypeptide acts as a potent stimulator of insulin secretion and a poor inhibitor of gastric acid secretion. Function: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Subcellular Location: Secreted. Similarity: Belongs to the glucagon family. SWISS: P09681 Gene ID: 2695 Database links: Entrez Gene: 2695 Human Omim: 137240 Human SwissProt: P09681 Human Unigene: 1454 Human 胃泌素抑制肽（GIP）是胃肠道的一种由42个氨基酸组成的多肽激素，又称为葡萄糖依赖性促胰岛素多肽（glucose-dependent insulinotropic peptide，GIP）他强烈抑制胃分泌和运动，也可调节胰岛素的释放。GIP属于Glucagon家族成员。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GIP) Polyclonal Antibody, Unconjugated (bs-0098R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GIP) polyclonal Antibody, Unconjugated (bs-0098R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
