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Rabbit Anti-GLUT3 antibody (bs-1207R)
~~~促销,代码KT802~~~
~~~促销,代码KT0830~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-1207R
英文名称 GLUT3
中文名称 葡萄糖转运蛋白3抗体
别    名 facilitated glucose transporter member 3; Glucose Transporter GLUT3 ; FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GTR3_HUMAN; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3; Solute carrier family 2.  
Specific References  (3)     |     bs-1207R has been referenced in 3 publications.
[IF=] Hu C et al. Placentae for Low Birth Weight Piglets Are Vulnerable to Oxidative Stress, Mitochondrial Dysfunction, and Impaired Angiogenesis. Oxid Med Cell Longev. 2020 May 25;2020:8715412.  WB ;  Pig.  
[IF=5.47] Yan, Yu-E., et al. ʺSignificant Reduction of the GLUT3 Level, but not GLUT1 Level, Was Observed in the Brain Tissues of Several Scrapie Experimental Animals and Scrapie-Infected Cell Lines.ʺ Molecular neurobiology (2013): 1-14.  WB ;  
[IF=3.166] Yun S et al. Effects of lead exposure on brain glucose metabolism and insulin signaling pathway in the hippocampus of rats. Toxicol Lett. 2019 Aug;310:23-30.  WB ;  Rat.  
研究领域 肿瘤  细胞生物  信号转导  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLUT3:151-260/493 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 may act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury. Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. Alternative names: FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT3; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3.

Function:
Facilitative glucose transporter. Probably a neuronal glucose transporter.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Melanosome.

Tissue Specificity:
Highly expressed in brain. Expressed in many tissues.

Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

SWISS:
P11169

Gene ID:
6515

Database links:

Entrez Gene: 6515 Human

Entrez Gene: 20527 Mouse

Entrez Gene: 25551 Rat

Omim: 138170 Human

SwissProt: P11169 Human

SwissProt: P32037 Mouse

SwissProt: Q07647 Rat

Unigene: 419240 Human

Unigene: 395108 Mouse

Unigene: 95055 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

交换和转运(Trafficking and Transport)
GLUT-3属于溶质运载蛋白家族成员(solute carrier family),主要功能是转载葡萄糖进入上皮细胞。
目前主要用于糖尿病肾病和视网膜病变的研究,也是肾小球系膜细胞上的主要葡萄糖转运体。GLUT3的功能状态直接影响系膜细胞的糖代谢及功能变化。
近期,研究人员也用来区别一些良、恶性肿瘤的鉴别。
产品图片
Sample:
293T(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-bs-1207R  (bs-1207R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 63 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
293T (Human) Cell Lysate at 30 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
Primary: Anti-GLUT3 (bs-1207R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 50/63 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, FITC conjugated(bs-1207R-FITC) 1:100, 60 minutes at 37°C;
Excitation wavelength: 488nm; Emission wavelength:519nm
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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