产品中心-北京博奥森生物技术有限公司

产品编号	bs-0384R
英文名称	GLUT4
中文名称	葡萄糖转运蛋白4抗体
别名	insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2, facilitated glucose transporter member 4.
	Specific References (29) \| bs-0384R has been referenced in 29 publications. [IF=8.025] Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113 WB ; Mouse. PubMed:35718141 [IF=6.78] Zhe Jiang. et al. RSL1D1 modulates cell senescence and proliferation via regulation of PPARγ mRNA stability. LIFE SCI. 2022 Aug;:120848 WB ; Human. PubMed:35940221 [IF=6.59] Yuge Jiang. et al. Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake.. FRONT NUTR. 2022 Nov;9:1039753-1039753 WB ; Mouse. PubMed:36424928 [IF=6.317] Zheng Ran. et al. Microbiome–metabolomics analysis reveals the potential effect of verbascoside in alleviating cognitive impairment in db/db mice. FOOD FUNCT. 2023 Mar;: WB ; Mouse. PubMed:37000613 [IF=5.988] Fu-Rong Yang. et al. Xiaoyaosan Exerts Antidepressant-Like Effect by Regulating Autophagy Involves the Expression of GLUT4 in the Mice Hypothalamic Neurons. FRONT PHARMACOL. 2022; 13: 873646 IF ; Mouse. PubMed:35784760 [IF=5.62] Ying-Ying Yang. et al. The human islet amyloid polypeptide reduces hippocampal tauopathy and behavioral impairments in P301S mice without inducing neurotoxicity or seeding amyloid aggregation. EXP NEUROL. 2023 Apr;362:114346 WB ; Mouse. PubMed:36750170 [IF=5.5] Sikder et al. High Fat Diet Upregulates Fatty Acid Oxidation and Ketogenesis via Intervention of PPAR-γ. (2018) Cell.Physiol.Biochem. 48:1317-1331 WB ; Mouse. PubMed:30048968 [IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB ; Mouse. PubMed:34706542 [IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB ; Mouse. PubMed:34706542 [IF=4.636] Cheng Wanpeng. et al. Transcriptomic analysis reveals the effects of maternal selenium deficiency on placental transport, hormone synthesis, and immune response in mice. METALLOMICS. 2022 Aug;: WB ; Mouse. PubMed:36002020 [IF=4.362] WB, IHC ; Mouse. PubMed:32973490 [IF=4.362] Zhang Yan-hui. et al. α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice. Front Aging Neurosci. 2020 Aug;12:262 WB ; Mouse. PubMed:32973490 [IF=4.221] Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192 WB ; Mouse. PubMed:35489422 [IF=4.221] Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192 WB ; Mouse. PubMed:35489422 [IF=3.998] Liza D. Morales. et al. Further evidence supporting a potential role for ADH1B in obesity. Sci Rep-Uk. 2021 Jan;11(1):1-14 WB ; Human. PubMed:33479282 [IF=3.871] Pengli Wang. et al. The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver. Mol Cell Endocrinol. 2020 Dec;518:111022 WB,IHC ; Rat. PubMed:32871226 [IF=3.69] Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947 WB ; Rat. PubMed:33617969 [IF=3.3] Zhang, Shihai, et al. "Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2." British Journal of Nutrition (2016): 1-10. WB ; Mouse, Pig. PubMed:27464458 [IF=2.659] Ye X et al. Irisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGC-1α pathway. Peptides. 2019 Jul 24:170120. WB ; Mouse. PubMed:31351089 [IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292 WB ; Rat. PubMed:36118100 [IF=2.629] Cao Zhanhong. et al. Protective Effects of Huangqi Shengmai Yin on Type 1 Diabetes-Induced Cardiomyopathy by Improving Myocardial Lipid Metabolism. Evid-Based Compl Alt. 2021;2021:5590623 WB ; Mouse. PubMed:34249132 [IF=2.302] Li Q et al. All-trans retinoic acid regulates sheep primary myoblasts proliferation and differentiation in vitro. Domestic Animal Endocrinology,2019 106394. WB ; Sheep. PubMed:doi:10.1016/j.domaniend.2019.106394 [IF=2.146] Xiaolan Wang. et al. The total flavonoids from Selaginella tamariscina (beauv.) Spring improve glucose and lipid metabolism in db/db mice. Iran J Basic Med Sci. 2020 Oct; 23(10): 1286–1292 WB ; Rat. PubMed:33149860 [IF=1.984] Pan LL et al. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine, 2019, 1–17. WB ; Rat. PubMed:doi:10.1155/2019/1394641 [IF=1.88] Shihe Zhang. et al. Astragalus polysaccharide regulates brown adipocytes differentiation by miR-6911 targeting Prdm16. 2021 Nov 05 WB ; Mouse. PubMed:34738642 [IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193. WB ; ram lambs. PubMed:31022943 [IF=1.58] Gao, Sujie, et al. "Propofol inhibits growth of neurons through regulating insulin receptor and insulin-like growth factor-1 receptor." Int J Clin Exp Pathol 9.7 (2016): 6785-6794. WB ; Rat. PubMed:ISSN:1936-2625/IJCEP0023819 [IF=0] Afolabi Oladele Ayobami. et al. Diet-induced insulin resistance altered cardiac GLUT4 and FATP/CD36 expression in rats. Beni-Suef University Journal of Basic and Applied Sciences. 2022 Dec;11(1):1-8 IHC ; Rat. PubMed:10.1186/s43088-022-00312-1 [IF=0] Song B et al. The effects of emodin on insulin resistance in KKAy mice with diabetes mellitus. Phcog Mag. 2018;14:344-50. IHC-P ; Mouse. PubMed:10.4103/pm.pm_362_17
研究领域	肿瘤心血管免疫学信号转导干细胞糖尿病
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Dog,Pig,Cow,Rabbit,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	54kDa
细胞定位	细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human GLUT4: 401-509/509 <Cytoplasmic>
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes. Function: Insulin-regulated facilitative glucose transporter. Subcellular Location: Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. Tissue Specificity: Skeletal and cardiac muscles; brown and white fat. Post-translational modifications: Sumoylated. DISEASE: Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects. Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. SWISS: P14672 Gene ID: 6517 Database links: Entrez Gene: 282359 Cow Entrez Gene: 6517 Human Entrez Gene: 20528 Mouse Entrez Gene: 25139 Rat Omim: 138190 Human SwissProt: Q27994 Cow SwissProt: Q29RP5 Cow SwissProt: P14672 Human SwissProt: P14142 Mouse SwissProt: P19357 Rat Unigene: 380691 Human Unigene: 10661 Mouse Unigene: 1314 Rat 葡萄糖转运蛋白-4是一种十分重要的葡萄糖转运体,与胰岛素抵抗和2型糖尿病密切相关.葡萄糖转运蛋白4在细胞内部和细胞膜之间循环流动.实现对葡萄糖的转运需要葡萄糖转运蛋白4自身的转位和活化.葡萄糖转运蛋白4与肥胖、肿瘤相关联. GLUT-2和GLUT-4蛋白这两个葡萄糖运载体的研究对于糖尿病的胰岛素释放障碍和胰岛素抵抗有重要意义.
产品图片	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- GLUT4 (bs-0384R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Heart(Rat) Cell Lysate at 40 ug Primary: Anti-GLUT4 (bs-0384R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: Muscle (Mouse) Lysate at 40 ug Lane 5: Muscle (Rat) Lysate at 40 ug Primary: Anti-GLUT4 (bs-0384R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): K562 (blue). Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (bs-0384R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记