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Rabbit Anti-GRP78 antibody (bs-1219R)
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-1219R
英文名称 GRP78
中文名称 葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体
别    名 BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.  
Specific References  (15)     |     bs-1219R has been referenced in 15 publications.
[IF=5.96] Xia, Dan, et al. "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress." Cell Death and Disease 8.7 (2017).  WB ;  Human.  
[IF=4.557] Zhao,et al.Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis.(2018) Immunology and Cell Biology. 96:401-412.  IF(IHC-F) ;  Mouse.  
[IF=4.302] Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2.   IHC-P ;  Human.  
[IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.  WB ;  Human.  
[IF=3.37] Du, Shaoqing, et al. "SelK is a novel ER stress-regulated protein and protects HepG2 cells from ER stress agent-induced apoptosis." Archives of biochemistry and biophysics 502.2 (2010): 137-143.  WB ;  Human.  
[IF=3.28] Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13.  WB ;  Mouse.  
[IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.   WB ;  Mouse.  
[IF=2.776] Su M et al.Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress. (2019) Environ Toxicol Pharmacol.65:60-65.   WB ;  Mouse.  
[IF=2.77] Zuo Y et al. Preoperative vitamin-rich carbohydrate loading alleviates postoperative cognitive dysfunction in aged rats. Behav Brain Res. 2019 Nov 5;373:112107.  WB ;  Rat.  
[IF=2.71] Wei, W, et al. "Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion." Environmental Toxicology 28 (2013):  Human.  
[IF=1.954] Jiang H et al.Strontium Alleviates Endoplasmic Reticulum Stress in a Nonalcoholic Fatty Liver Disease Model.(2018)J Med Food. Nov 17.   IHC ;  Rat.  
[IF=1.39] Tian et al. Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma. (2017) Oncol.Let. 13:1437-1443  WB ;  Human.  
[IF=0] Du, Weiyan, et al. "The Expression and Significance of Angiopoietin-2 and Glucose Regulated Protein78 in Colorectal Cancer." Cancer Cell Research (2016).  IHC-P ;  Human.  
[IF=0] Tian, Jing, Rong Liu, and Quanxin Qu. "Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma." Oncology Letters.  WB ;  Human.  
[IF=0] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742.   WB ;  Mouse.  
研究领域 肿瘤  细胞生物  免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 78kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GRP78:251-360/654 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Function:
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

DISEASE:
Note=Autoantigen in rheumatoid arthritis.

Similarity:
Belongs to the heat shock protein 70 family.

SWISS:
P11021

Gene ID:
3309

Database links:

Entrez Gene: 3309 Human

Entrez Gene: 14828 Mouse

Entrez Gene: 25617 Rat

Omim: 138120 Human

SwissProt: P11021 Human

SwissProt: P20029 Mouse

SwissProt: P06761 Rat

Unigene: 605502 Human

Unigene: 743241 Human

Unigene: 330160 Mouse

Unigene: 470180 Mouse

Unigene: 11088 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

葡萄糖调节蛋白78是热休克蛋白70家族的成员之一,又称:热休克蛋白70蛋白5,作为一种分子伴侣在蛋白质的折叠和转运过程及内质网应激反应中发挥重要作用.它在多种肿瘤组织中呈高表达.
产品图片
Sample: Intestinal(Mouse) Lysate at 30ug;
Primary: Anti-GRP78 (bs-1219R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 78 kD
Observed band size :78 kD
Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: U-87MG (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Hyperpituitarism (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Sample:
U87mg(Human) Cell Lysate at 30 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(bs-1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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