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Rabbit Anti-HMGB1  antibody (bs-0664R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0664R
英文名称 HMGB1
中文名称 高迁移率族蛋白B1抗体
别    名 High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.  
Specific References  (28)     |     bs-0664R has been referenced in 28 publications.
[IF=15.881] Yuting Shen. et al. Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation. Acs Nano. 2021;XXXX(XXX):XXX-XXX  IF ;  Mouse.  
[IF=15.621] Feng B et al. Enhancing Triple Negative Breast Cancer Immunotherapy by ICG‐Templated Self‐Assembly of Paclitaxel Nanoparticles. Advanced Functional Materials,2019 1906605.  FCM&ICC ;  Mouse.  
[IF=15.153] Songlin Gong. et al. Tumor Microenvironment-Activated Hydrogel Platform with Programmed Release Property Evokes a Cascade-Amplified Immune Response against Tumor Growth, Metastasis and Recurrence. SMALL. 2022 Nov;:2107061  IF ;  Mouse.  
[IF=12.13] Tian Zhang. et al. Supramolecular Tadalafil Nanovaccine for Cancer Immunotherapy by Alleviating Myeloid-Derived Suppressor Cells and Heightening Immunogenicity. 2021 May 13  IHC ;  Mouse.  
[IF=10.383] Qingfei Zhang. et al. Hierarchical Microparticles Delivering Oxaliplatin and NLG919 Nanoprodrugs for Local Chemo-immunotherapy. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXX  IF ;  Mouse.  
[IF=9.038] Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:124050  WB,IF,IP ;  Human.  
[IF=8.947] Zhen-Han Feng. et al. A combination strategy based on an Au nanorod/doxorubicin gel via mild photothermal therapy combined with antigen-capturing liposomes and anti-PD-L1 agent promote a positive shift in the cancer-immunity cycle. Acta Biomater. 2021 Oct;:  IF ;  Mouse.  
[IF=6.304] Chen Y et al. Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414.  WB ;  Mouse.  
[IF=5.295] Xiahong Tang. et al. Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 May;:  WB ;  Rat.  
[IF=3.687] Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10.  WB ;  Rat.  
[IF=3.549] Hong et al. Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats. (2018) Mediators.Inflamm. 2017:9783893  IHC ;  Rat.  
[IF=3.457] Ling L et al. MicroRNA-30e promotes hepatocyte proliferation and inhibits apoptosis in cecal ligation and puncture-induced sepsis through the JAK/STAT signaling pathway by binding to FOSL2.Biomed Pharmacother. 2018 Aug;104:411-419.  WB ;  Rat.  
[IF=3.337] Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759–7771  WB ;  Human.  
[IF=3.24] Zhao, Lu, et al. "The high mobility group box 1 protein of< i> Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation." Developmental & Comparative Immunology 35.10 (2011): 1052-1058.  WB ;  
[IF=3.166] Shi Q et al. Polychlorinated biphenyl quinone-induced signaling transition from autophagy to apoptosis is regulated by HMGB1 and p53 in human hepatoma HepG2 cells. Toxicol Lett. 2019 May 15;306:25-34.  WB,Co-IP&IF ;  Human.  
[IF=3.12] Jiang, W., et al. "Curculigoside A attenuates experimental cerebral ischemia injury< i> in vitro and< i> vivo." Neuroscience 192 (2011): 572-579.  WB ;  Rat.  
[IF=3.05] Rui, Zhang, et al. "Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model." Nutrition (2014).  WB ;  Rat.  
[IF=2.996] Xiang Ao. et al. Lavender essential oil accelerates lipopolysaccharide-induced chronic wound healing by inhibiting caspase-11-mediated macrophage pyroptosis. KAOHSIUNG J MED SCI. 2023 Feb;:  WB ;  Mouse.  
[IF=2.886] Lei Chen. et al. Circ_0032821 Facilitates Gastric Cancer Cell Proliferation, Migration, Invasion and Glycolysis by Regulating MiR-1236-3p/HMGB1 Axis. Cancer Manag Res. 2020; 12: 9965–9976  WB ;  Human.  
[IF=2.766] Xiang et al. Glycyrrhizin suppresses the expressions of HMGB1 and relieves the severity of traumatic pancreatitis in rats. (2014) PLoS.One. 9:e115982  IHC ;  Rat.  
[IF=2.61] Li, Yuan-bo, et al. "Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression." Molecular and Cellular Biochemistry: 1-10.  Human.  
[IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775.  WB ;  Rat.  
[IF=2.341] Diao S et al. IGF2 enhanced the osteo‐/dentinogenic and neurogenic differentiation potentials of stem cells from apical papilla. J Oral Rehabil. 2019 Jul 10.  WB ;  Human.  
[IF=1.89] Sun et al. Expression and Significance of High-Mobility Group Protein B1 (HMGB1) and the Receptor for Advanced Glycation End-Product (RAGE) in Knee Osteoarthritis. (2016) Med.Sci.Monit. 22:2105-12  IHC,WB ;  Human.  
[IF=1.34] Sun, Shanping, et al. "High mobility group box-1 and its clinical value in breast cancer." OncoTargets and Therapy 8 (2015): 413.  IHC-P ;  Human.  
[IF=1.075] Qiao et al. Effect of different 1, 25-(OH)2D3 doses on high mobility group box1 and toll-like receptors 4 expression in lung tissue of asthmatic mice. (2015) Int.J.Clin.Exp.Med. 8:4016-23  IHC ;  Mouse.  
[IF=0.94] Wang, Xin‑Jun, et al. "Clinical and prognostic significance of high-mobility group box-1 in human gliomas." Experimental and Therapeutic Medicine.  WB ;  Human.  
[IF=0] He et al. The effects of n-3 PUFA and intestinal lymph drainage on high-mobility group box 1 and Toll-like receptor 4 mRNA in rats with intestinal ischaemia-reperfusion injury. (2012) Br.J.Nut. 108:883-92  WB, IHC-P ;  Rat.  
研究领域 肿瘤  细胞生物  免疫学  转录调节因子  结合蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human (predicted: Cow)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 25kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HMGB1: 75-170/215 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models

Function:
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Subunit:
Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2.

Subcellular Location:
Nucleus. Chromosome.

Similarity:
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.

SWISS:
P09429

Gene ID:
3146

Database links:

Entrez Gene: 282691 Cow

Entrez Gene: 3146 Human

Entrez Gene: 100862258 Mouse

Entrez Gene: 15289 Mouse

Entrez Gene: 25459 Rat

Omim: 163905 Human

SwissProt: P10103 Cow

SwissProt: P09429 Human



近来的研究表明称之为高迁移率族蛋白B-1(HMG-B1)的核内结构蛋白在核外表达时是一种有效的早期炎症介质。
高迁移性B1组蛋白(HMGB1): 是一种核结合蛋白,在DNA重组、修复、复制和基因转录中起作用。HMGB1也是巨噬细胞分泌的一种介质。
此外,高迁移性B1组蛋白亦被受刺激的巨噬细胞或单核细胞主动分泌。在这一主动分泌过程中,HMGB1首先经乙酰化并由核内转移至溶酶体内,继而在ATP和溶血磷脂胆碱两种分泌信号指导下转移至细胞外。由坏死细胞被动释放的HMGB1和炎症细胞主动分泌的HMGB1存在分子上的差异。胞外的HMGB1可作为细胞因子参与信号传导,因为它既可识别Toll 样受体(TLR)家族的一些成员,又能与识别晚期糖基化终末产物受体(RAGE)相作用。
HMGB1能启动炎症反应,包括产生多种细胞因子、对某些干细胞产生趋化作用、诱导血管粘附分子、削弱肠上皮细胞的功能等。
产品图片
Sample: bone (mouse) Lysate at 40 ug
Primary: Anti- HMGB1(bs-0664R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27kD
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGB1 Polyclonal Antibody, Unconjugated(bs-0664R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGB1 Polyclonal Antibody, Unconjugated(bs-0664R) 1:400, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HMGB1) polyclonal Antibody, Unconjugated (bs-0664R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-0664R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-0664R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Rat splenocytes stained with Anti- HMGB1 Polyclonal Antibody, A488 Conjugated (bs-0664R-A488) at 1:50.
The figure annotation:
The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC) The green histogram is cells stained with Rabbit Anti-HMGB1/FITC Conjugated antibody (bs-0664R-FITC).
Controls
Positive control: HepG 2 cells Isotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC). instead of the primary antibody to confirm that primary antibody binding is specific. 5μgin 100 μL 1 X PBS containing 0.5% BSA.
Positive control: HepG2 cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
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